Characterization of an antimicrobial and phytotoxic ribonuclease secreted by the fungal wheat pathogen Zymoseptoria tritici.
Characterization of an antimicrobial and phytotoxic ribonuclease secreted by the fungal wheat pathogen Zymoseptoria tritici.
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DOI：
10.1111/nph.14786
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发表时间：
2018-01
期刊：
影响因子：
--
通讯作者：
Rudd JJ
中科院分区：
文献类型：
--
作者：
Kettles GJ;Bayon C;Sparks CA;Canning G;Kanyuka K;Rudd JJ
小麦发酵酵母菌是小麦叶壳针孢斑病 (STB) 病的病原体。小麦发酵斑孢菌在感染过程中分泌许多功能上未表征的效应蛋白。在这里，我们表征了一种具有不寻常的双相表达模式的分泌性核糖核酸酶（Zt6）。使用瞬时表达系统来表征宿主和非宿主植物中的 Zt6 及其突变体。无细胞蛋白表达系统监测 Zt6 蛋白对功能性核糖体的影响，并对用重组 Zt6 处理的细胞进行体外测定，确定对细菌、酵母和丝状真菌的毒性。我们证明了 Zt6 是一种功能性核糖核酸酶，其植物毒性取决于 22 个氨基酸 N 末端“环”区域的存在及其催化活性。 Zt6 选择性切割植物和动物 rRNA 物种，对小麦、烟草、细菌和酵母细胞有毒，但对小麦小麦本身没有毒性。 Zt6 是第一个被证明可能具有双重功能的小麦小麦效应器。 Zt6 的表达模式和对微生物的强毒性表明，尽管它可能有助于执行小麦细胞死亡，但它也可能在抗菌竞争和生态位保护中具有重要的次要功能。
The fungus Zymoseptoria tritici is the causal agent of Septoria Tritici Blotch (STB) disease of wheat leaves. Zymoseptoria tritici secretes many functionally uncharacterized effector proteins during infection. Here, we characterized a secreted ribonuclease (Zt6) with an unusual biphasic expression pattern. Transient expression systems were used to characterize Zt6, and mutants thereof, in both host and non‐host plants. Cell‐free protein expression systems monitored the impact of Zt6 protein on functional ribosomes, and in vitro assays of cells treated with recombinant Zt6 determined toxicity against bacteria, yeasts and filamentous fungi. We demonstrated that Zt6 is a functional ribonuclease and that phytotoxicity is dependent on both the presence of a 22‐amino‐acid N‐terminal ‘loop’ region and its catalytic activity. Zt6 selectively cleaves both plant and animal rRNA species, and is toxic to wheat, tobacco, bacterial and yeast cells, but not to Z. tritici itself. Zt6 is the first Z. tritici effector demonstrated to have a likely dual functionality. The expression pattern of Zt6 and potent toxicity towards microorganisms suggest that, although it may contribute to the execution of wheat cell death, it is also likely to have an important secondary function in antimicrobial competition and niche protection.
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影响因子：
6.7
作者：
Liu Z;Zhang Z;Faris JD;Oliver RP;Syme R;McDonald MC;McDonald BA;Solomon PS;Lu S;Shelver WL;Xu S;Friesen TL
通讯作者：
Friesen TL
影响因子：
14.8
作者：
Kelley LA;Mezulis S;Yates CM;Wass MN;Sternberg MJ
通讯作者：
Sternberg MJ
影响因子：
4.4
作者：
Pedersen C;Ver Loren van Themaat E;McGuffin LJ;Abbott JC;Burgis TA;Barton G;Bindschedler LV;Lu X;Maekawa T;Wessling R;Cramer R;Thordal-Christensen H;Panstruga R;Spanu PD
通讯作者：
Spanu PD
影响因子：
3
作者：
Ben M'Barek, Sarrah;Cordewener, Jan H. G.;Kema, Gerrit H. J.
通讯作者：
Kema, Gerrit H. J.
影响因子：
3.6
作者：
Bolton, Melvin D.;van Esse, H. Peter;Thomma, Bart P. H. J.
通讯作者：
Thomma, Bart P. H. J.