Gibberellin and auxin influence the diurnal transcription pattern of photoreceptor genes via CRY1a in tomato.
Gibberellin and auxin influence the diurnal transcription pattern of photoreceptor genes via CRY1a in tomato.
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DOI：
10.1371/journal.pone.0030121
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Perrotta G
中科院分区：
文献类型：
--
作者：
Facella P;Daddiego L;Giuliano G;Perrotta G
植物光感受器，光敏色素和隐花色素，调节发育和生长的许多方面，如种子萌发、茎伸长、幼苗去黄化、子叶开放、花诱导和昼夜节律。在所有这些生理过程中，光感受器和植物激素之间的相互作用有几个证据，但对光感受器-光感受器串扰的分子和遗传机制知之甚少。在这项工作中，我们研究了外源植物激素的分子效应，光感受器基因转录番茄野生型，以及转基因和突变系改变隐花色素，通过监测白天/夜间转录振荡。GA和生长素改变番茄中不同光感受器基因的昼夜表达水平，特别是在缺乏隐花色素1a的工作形式的突变体中：在这些突变体中，一些（IAA）或大多数（GA）光感受器基因的表达被这些激素下调。我们的研究结果突出了隐花色素1a蛋白，激素和光感受器的基因表达在番茄中的分子关系的存在，这表明隐花色素的操作可以代表一个很好的策略，以更深入地了解植物激素在植物光感知机制中的作用。
Plant photoreceptors, phytochromes and cryptochromes, regulate many aspects of development and growth, such as seed germination, stem elongation, seedling de-etiolation, cotyledon opening, flower induction and circadian rhythms. There are several pieces of evidence of interaction between photoreceptors and phyto-hormones in all of these physiological processes, but little is known about molecular and genetic mechanisms underlying hormone-photoreceptor crosstalk. In this work, we investigated the molecular effects of exogenous phyto-hormones to photoreceptor gene transcripts of tomato wt, as well as transgenic and mutant lines with altered cryptochromes, by monitoring day/night transcript oscillations. GA and auxin alter the diurnal expression level of different photoreceptor genes in tomato, especially in mutants that lack a working form of cryptochrome 1a: in those mutants the expression of some (IAA) or most (GA) photoreceptor genes is down regulated by these hormones. Our results highlight the presence of molecular relationships among cryptochrome 1a protein, hormones, and photoreceptors' gene expression in tomato, suggesting that manipulation of cryptochromes could represent a good strategy to understand in greater depth the role of phyto-hormones in the plant photoperceptive mechanism.
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影响因子：
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