A terrified-sound stress induced proteomic changes in adult male rat hippocampus
A terrified-sound stress induced proteomic changes in adult male rat hippocampus
复制标题
恐怖声音应激诱导成年雄性大鼠海马蛋白质组变化
DOI：
10.1016/j.physbeh.2014.01.038
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发表时间：
2014-04
期刊：
影响因子：
--
通讯作者：
Chen Huang
中科院分区：
文献类型：
--
作者：
Lingyu Zhao;Xiaoge Zhao;Tusheng Song;Chen Huang
在这项研究中，我们研究了成年大鼠海马体中恐惧声音引起的心理压力与空间学习之间关系的生化机制。成年雄性大鼠受到恐怖声音应激，莫里斯水迷宫（MWM）已被用来评估空间学习和记忆的变化。使用二维凝胶电泳（2DE）、基质辅助激光解吸/电离飞行时间质谱和生物信息学分析检查海马的蛋白质表达谱。 MWM 测试的数据表明，恐惧的声音压力可以改善空间学习。蛋白质组分析显示，应激大鼠海马中有52种蛋白表达下调，35种蛋白表达上调。我们鉴定并验证了六种最显着的差异表达蛋白质，这些蛋白质表现出最大的压力诱导变化。我们的研究提供了第一个证据，证明恐惧声音压力可以改善大鼠的空间学习，并且空间学习的增强与大鼠海马体中蛋白质表达的变化相一致。
In this study, we investigated the biochemical mechanisms in the adult rat hippocampus underlying the relationship between a terrified-sound induced psychological stress and spatial learning. Adult male rats were exposed to a terrified-sound stress, and the Morris water maze (MWM) has been used to evaluate changes in spatial learning and memory. The protein expression profile of the hippocampus was examined using two-dimensional gel electrophoresis (2DE), matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, and bioinformatics analysis. The data from the MWM tests suggested that a terrified-sound stress improved spatial learning. The proteomic analysis revealed that the expression of 52 proteins was down-regulated, while that of 35 proteins were up-regulated, in the hippocampus of the stressed rats. We identified and validated six of the most significant differentially expressed proteins that demonstrated the greatest stress-induced changes. Our study provides the first evidence that a terrified-sound stress improves spatial learning in rats, and that the enhanced spatial learning coincides with changes in protein expression in rat hippocampus.
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影响因子：
9.8
作者：
Kuiper JW;Pluk H;Oerlemans F;van Leeuwen FN;de Lange F;Fransen J;Wieringa B
通讯作者：
Wieringa B
影响因子：
3.7
作者：
Zuena AR;Mairesse J;Casolini P;Cinque C;Alemà GS;Morley-Fletcher S;Chiodi V;Spagnoli LG;Gradini R;Catalani A;Nicoletti F;Maccari S
通讯作者：
Maccari S
影响因子：
2.7
作者：
Astur, RS;Taylor, LB;Sutherland, RJ
通讯作者：
Sutherland, RJ
影响因子：
4.2
作者：
Liu, Yanyong;Yang, Nan;Zuo, Pingping
通讯作者：
Zuo, Pingping
影响因子：
6
作者：
Yang, PC;Jury, J;Perdue, MH
通讯作者：
Perdue, MH