Neonatal systemic inflammation in rats alters retinal vessel development and simulates pathologic features of retinopathy of prematurity.
Neonatal systemic inflammation in rats alters retinal vessel development and simulates pathologic features of retinopathy of prematurity.
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DOI：
10.1186/1742-2094-11-87
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发表时间：
2014-05-15
影响因子：
9.3
通讯作者：
Oh JY
中科院分区：
文献类型：
--
作者：
Hong HK;Lee HJ;Ko JH;Park JH;Park JY;Choi CW;Yoon CH;Ahn SJ;Park KH;Woo SJ;Oh JY
Alteration of retinal angiogenesis during development leads to retinopathy of prematurity (ROP) in preterm infants, which is a leading cause of visual impairment in children. A number of clinical studies have reported higher rates of ROP in infants who had perinatal infections or inflammation, suggesting that exposure of the developing retina to inflammation may disturb retinal vessel development. Thus, we investigated the effects of systemic inflammation on retinal vessel development and retinal inflammation in neonatal rats. To induce systemic inflammation, we intraperitoneally injected 100 μl lipopolysaccharide (LPS, 0.25 mg/ml) or the same volume of normal saline in rat pups on postnatal days 1, 3, and 5. The retinas were extracted on postnatal days 7 and 14, and subjected to assays for retinal vessels, inflammatory cells and molecules, and apoptosis. We found that intraperitoneal injection of LPS impaired retinal vessel development by decreasing vessel extension, reducing capillary density, and inducing localized overgrowth of abnormal retinal vessels and dilated peripheral vascular ridge, all of which are characteristic findings of ROP. Also, a large number of CD11c+ inflammatory cells and astrocytes were localized in the lesion of abnormal vessels. Further analysis revealed that the number of major histocompatibility complex (MHC) class IIloCD68loCD11bloCD11chi cells in the retina was higher in LPS-treated rats compared to controls. Similarly, the levels of TNF-α, IL-1β, and IL-12a were increased in LPS-treated retina. Also, apoptosis was increased in the inner retinal layer where retinal vessels are located. Our data demonstrate that systemic LPS-induced inflammation elicits retinal inflammation and impairs retinal angiogenesis in neonatal rats, implicating perinatal inflammation in the pathogenesis of ROP.
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影响因子：
168.9
作者：
Mwaniki, Michael K.;Atieno, Maurine;Lawn, Joy E.;Newton, Charles R. J. C.
通讯作者：
Newton, Charles R. J. C.
影响因子：
3.7
作者：
Cardoso FL;Kittel A;Veszelka S;Palmela I;Tóth A;Brites D;Deli MA;Brito MA
通讯作者：
Brito MA
影响因子：
8
作者：
Chen ML;Guo L;Smith LE;Dammann CE;Dammann O
通讯作者：
Dammann O
影响因子：
9.8
作者：
Chen, Jing;Smith, Lois E. H.
通讯作者：
Smith, Lois E. H.
影响因子：
82.9
作者：
Kermorvant-Duchemin, E;Sennlaub, F;Chemtob, S
通讯作者：
Chemtob, S