Fractional Gluconeogenesis: A Biomarker of Dietary Energy Adequacy in a Rat Brain Injury Model.
Fractional Gluconeogenesis: A Biomarker of Dietary Energy Adequacy in a Rat Brain Injury Model.
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DOI：
10.3390/metabo12121163
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发表时间：
2022-11-23
期刊：
影响因子：
4.1
通讯作者：
Brooks GA
中科院分区：
文献类型：
--
作者：
Curl CC;Kumar A;Peck AJ;Arevalo JA;Gleason A;Leija RG;Osmond AD;Duong JJ;Miller BF;Horning MA;Brooks GA
创伤性脑损伤（TBI）治疗的患者由于创伤和营养不良而处于代谢危机中。我们利用部分再生（fGNG）来评估TBI后自由进食和热量限制大鼠的营养充足性。将单独饲养的49日龄雄性Sprague-Dawley大鼠随机分为四组：自由采食（AL）对照组（AL-Con，假手术组）、AL + TBI（AL+TBI）、热量限制（CR）对照组（CR-Con，假手术组）和CR + TBI（CR+TBI）。从第1-7天，动物被给予AL接触含有6%氧化氘（D2 O）的食物和水。在第8天，从每只动物抽取干预前血液样品，并开始TBI、假损伤和CR方案。在第22天，将动物安乐死，并收集血液以测量fGNG。干预前，各组之间的fGNG无显著差异（p ≥ 0.05）。热量限制导致fGNG显著增加，与TBI无关（p ≤ 0.05）。此外，fGNG可以提供用于评估患者饮食热量需求的实时、个性化生物标志物。
Patients treated for traumatic brain injury (TBI) are in metabolic crises because of the trauma and underfeeding. We utilized fractional gluconeogenesis (fGNG) to assess nutritional adequacy in ad libitum-fed and calorically-restricted rats following TBI. Male Sprague–Dawley individually housed rats 49 days of age were randomly assigned into four groups: ad libitum (AL) fed control (AL-Con, sham), AL plus TBI (AL+TBI), caloric restriction (CR) control (CR-Con, sham), and CR plus TBI (CR+TBI). From days 1–7 animals were given AL access to food and water containing 6% deuterium oxide (D2O). On day 8, a pre-intervention blood sample was drawn from each animal, and TBI, sham injury, and CR protocols were initiated. On day 22, the animals were euthanized, and blood was collected to measure fGNG. Pre-intervention, there was no significant difference in fGNG among groups (p ≥ 0.05). There was a significant increase in fGNG due to caloric restriction, independent of TBI (p ≤ 0.05). In addition, fGNG may provide a real-time, personalized biomarker for assessing patient dietary caloric needs.
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DOI：
10.1093/function/zqab028
发表时间：
2021
期刊：
Function (Oxford, England)
影响因子：
--
作者：
Abbott CB;Lawrence MM;Kobak KA;Lopes EBP;Peelor FF 3rd;Donald EJ;Van Remmen H;Griffin TM;Miller BF
通讯作者：
Miller BF
影响因子：
4.2
作者：
Glenn TC;Martin NA;McArthur DL;Hovda DA;Vespa P;Johnson ML;Horning MA;Brooks GA
通讯作者：
Brooks GA
影响因子：
3.3
作者：
Chacko, Shaji K.;Sunehag, Agneta L.;Haymond, Morey W.
通讯作者：
Haymond, Morey W.
DOI：
10.1073/pnas.4.12.370
发表时间：
1918-01-01
影响因子：
11.1
作者：
Harris, JA;Benedict, FG
通讯作者：
Benedict, FG
影响因子：
38.9
作者：
Moinard, C;Neveux, N;Cynober, L
通讯作者：
Cynober, L