Wild primate microbiomes prevent weight gain in germ-free mice.
Wild primate microbiomes prevent weight gain in germ-free mice.
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DOI：
10.1186/s42523-020-00033-9
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发表时间：
2020-05-07
影响因子：
4.7
通讯作者：
Clayton JB
中科院分区：
文献类型：
--
作者：
Sidiropoulos DN;Al-Ghalith GA;Shields-Cutler RR;Ward TL;Johnson AJ;Vangay P;Knights D;Kashyap PC;Xian Y;Ramer-Tait AE;Clayton JB
肠道微生物组含有数万亿的细菌，这些细菌在膳食营养素提取和宿主代谢中起着重要作用。肥胖和糖尿病等代谢性疾病与微生物组组成的变化有关，并且在西方化或高度工业化的国家中呈上升趋势。与此同时，膳食纤维含量低的西式饮食已被证明会导致肠道微生物多样性的丧失。然而，微生物组组成，膳食纤维损失和肥胖之间的联系尚未得到很好的定义。为了研究肠道微生物群，膳食纤维和体重增加之间的相互作用，我们将圈养和野生douc肠道微生物群移植到无菌小鼠中，然后将它们暴露于高纤维或低纤维饮食。无论饮食如何，接受圈养douc微生物群的小鼠体重增加明显更多，而接受高纤维饮食和野生douc微生物群的小鼠保持苗条。在低纤维饮食的情况下，野生douc微生物群部分阻止了体重增加。使用16S rRNA基因扩增子测序，我们确定了每组中的关键细菌分类群，特别是捕获douc FMT小鼠中的拟杆菌属和阿克曼氏菌属的相对丰度较高，野生douc FMT小鼠中的乳杆菌属和梭菌属的相对丰度较高。在我们的无菌小鼠实验中，野生douc微生物群可以作为跨物种移植的微生物库。我们的研究结果表明，野生douc微生物群适合于不同的纤维饮食，并且可以在暴露于天然饮食时防止体重增加。
The gut microbiome harbors trillions of bacteria that play a major role in dietary nutrient extraction and host metabolism. Metabolic diseases such as obesity and diabetes are associated with shifts in microbiome composition and have been on the rise in Westernized or highly industrialized countries. At the same time, Westernized diets low in dietary fiber have been shown to cause loss of gut microbial diversity. However, the link between microbiome composition, loss of dietary fiber, and obesity has not been well defined. To study the interactions between gut microbiota, dietary fiber, and weight gain, we transplanted captive and wild douc gut microbiota into germ-free mice and then exposed them to either a high- or low-fiber diet. The group receiving captive douc microbiota gained significantly more weight, regardless of diet, while mice receiving a high-fiber diet and wild douc microbiota remained lean. In the presence of a low-fiber diet, the wild douc microbiota partially prevented weight gain. Using 16S rRNA gene amplicon sequencing we identified key bacterial taxa in each group, specifically a high relative abundance of Bacteroides and Akkermansia in captive douc FMT mice and a higher relative abundance of Lactobacillus and Clostridium in the wild douc FMT mice. In the context of our germ-free mouse experiment, wild douc microbiota could serve as a reservoir for microbes for cross-species transplants. Our results suggest that wild douc microbiota are tailored to diverse fiber diets and can prevent weight gain when exposed to a native diet.
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美国移民对人类肠道微生物组进行了西化。
影响因子：
64.5
作者：
Vangay P;Johnson AJ;Ward TL;Al-Ghalith GA;Shields-Cutler RR;Hillmann BM;Lucas SK;Beura LK;Thompson EA;Till LM;Batres R;Paw B;Pergament SL;Saenyakul P;Xiong M;Kim AD;Kim G;Masopust D;Martens EC;Angkurawaranon C;McGready R;Kashyap PC;Culhane-Pera KA;Knights D
通讯作者：
Knights D
影响因子：
6.4
作者：
Al-Ghalith, Gabriel A.;Hillmann, Benjamin;Knights, Dan
通讯作者：
Knights, Dan
DOI：
10.1073/pnas.1219451110
发表时间：
2013-05-28
影响因子：
11.1
作者：
Everard, Amandine;Belzer, Clara;Cani, Patrice D.
通讯作者：
Cani, Patrice D.
定义生态失调及其对宿主免疫和疾病的影响。
影响因子：
3.4
作者：
Petersen C;Round JL
通讯作者：
Round JL
影响因子：
64.5
作者：
Goodrich JK;Waters JL;Poole AC;Sutter JL;Koren O;Blekhman R;Beaumont M;Van Treuren W;Knight R;Bell JT;Spector TD;Clark AG;Ley RE
通讯作者：
Ley RE