Manipulation of Bioparticles on Electrodeless Dielectrophoretic Chip Based on AC Electrokinetic Control
Manipulation of Bioparticles on Electrodeless Dielectrophoretic Chip Based on AC Electrokinetic Control
复制标题
基于交流动电控制的无电极介电泳芯片上的生物粒子操控
DOI：
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发表时间：
2007
期刊：
影响因子：
--
通讯作者：
Chi
中科院分区：
文献类型：
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作者：
Hsien;Chao;I. Cheng;Chi
设计了一种无电极介电泳（EDEP）芯片，用于分离不同粒径的微颗粒，并对实际样品的生物分离能力进行了检验。介电泳（DEP）力主要可以通过提供一个非均匀的电场来产生和控制，该电场由绝缘体图案芯片在几何上收缩，并结合频率为10 kHz和500 Vp-p的交流（AC）电场设置在通道入口两侧。EDEP芯片通过良好的DEP力控制，从人全血样品中分离大肠杆菌和红细胞。结果表明，细菌和红细胞可以在几秒内分别被分离到EDEP芯片的高电场区和低电场区。基于EDEP方法的快速、实用的诊断工具可应用于生物工业技术、临床感染的检测和鉴定等各个领域。
An electrodeless dielectrophoretic (EDEP) chip was designed and applied to separate the micro-particles in different sizes, and the ability of bio-separation of real samples also be examined. The dielectrophoretic (DEP) force can be principally created and controlled by provide a non-uniform electric field that is geometrically constricted by the insulator-patterned chip in combination with an alternative current (AC) electric field at frequency 10 kHz and 500 Vp-p set on the two sides of channel inlet. The EDEP chip was used to separate the E. coli and red blood cells (RBC), which from human whole blood sample, via well control of DEP force. Our results showed the bacteria and RBC can be separated into the higher and lower electric field regions of the EDEP chip in few seconds, respectively. A rapid, useful diagnosis tool, based on the EDEP method could be applied and used in the various fields of the bio-industry technology, the detection and the identification of clinical infections.
影响因子：
3.4
作者：
Chou, CF;Tegenfeldt, JO;Austin, RH
通讯作者：
Austin, RH