The programmed DNA elimination and formation of micronuclei in germ line cells of the natural hybridogenetic water frog Pelophylax esculentus.
The programmed DNA elimination and formation of micronuclei in germ line cells of the natural hybridogenetic water frog Pelophylax esculentus.
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DOI：
10.1038/s41598-018-26168-z
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发表时间：
2018-05-18
影响因子：
4.6
通讯作者：
Ogielska M
中科院分区：
文献类型：
--
作者：
Chmielewska M;Dedukh D;Haczkiewicz K;Rozenblut-Kościsty B;Kaźmierczak M;Kolenda K;Serwa E;Pietras-Lebioda A;Krasikova A;Ogielska M
DNA消除是基因沉默的一种根本形式，发生在体细胞和生殖细胞中。程序性DNA消除发生在种间杂种（竹节虫、鱼类和两栖动物）的配子发生过程中，这些种间杂种通过杂交生殖进行繁殖，并涉及去除一个亲本物种的整个基因组，并产生繁殖另一个物种基因组的克隆配子。细胞机制在杂交昆虫和鱼类中有很大不同，但在食用蛙Pelophylax esculentus中仍然未知，Pelophylax lessonae和Pelophylax ridibundus之间的天然杂交种。本文报道了用透射电镜、免疫荧光和细胞化学方法研究二倍体和三倍体杂交蛙性腺发育早期DNA消除机制的结果。在两性生殖细胞（初级卵原细胞和前精原细胞）中，微核以分裂间期形成的分离核芽的形式出现。我们发现，耗尽核孔复合物的微核膜和染色质失活，通过异染色质化，然后通过自噬降解微核。细胞核的形成不会导致凋亡性细胞死亡，这表明基因组消除是一个生理过程。在杂交遗传动物中，通过微核消除黄颡鱼的染色质是独特的，有助于拓宽对动物生殖模式的认识。
DNA elimination is a radical form of gene silencing and occurs both in somatic and germ cells. The programmed DNA elimination occurs during gametogenesis in interspecies hybrids that reproduce by hybridogenesis (stick insects, fishes, and amphibians) and concerns removal of whole genomes of one of the parental species and production of clonal gametes propagating the genome of the other species. The cellular mechanisms differ considerably in hybridogenetic insects and fishes but remains unknown in edible frogs Pelophylax esculentus, natural hybrids between Pelophylax lessonae and Pelophylax ridibundus. Here we report DNA elimination mechanism in early developing gonads of diploid and triploid hybrid frogs, studied by TEM, immunofluorescence, and cytochemistry. In gonocytes of both sexes (primary oogonia and prespermatogonia), micronuclei emerge as detached nuclear buds formed during interphase. We found depletion of nuclear pore complexes in micronuclear membrane and chromatin inactivation via heterochromatinization followed by degradation of micronuclei by autophagy. Micronuclei formation does not lead to apoptotic cell death showing that genome elimination is a physiological process. Chromatin elimination via micronuclei in P. esculentus is unique among hybridogenetic animals and contributes to broadening the knowledge about reproductive modes in animals.
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影响因子：
3.7
作者：
Dedukh, Dmitry;Litvinchuk, Spartak;Krasikova, Alla
通讯作者：
Krasikova, Alla
影响因子：
13.3
作者：
Fujiwara Y;Kikuchi H;Aizawa S;Furuta A;Hatanaka Y;Konya C;Uchida K;Wada K;Kabuta T
通讯作者：
Kabuta T
影响因子：
0.9
作者：
Haczkiewicz, Katarzyna;Ogielska, Maria
通讯作者：
Ogielska, Maria
影响因子：
2.9
作者：
Dedukh D;Mazepa G;Shabanov D;Rosanov J;Litvinchuk S;Borkin L;Saifitdinova A;Krasikova A
通讯作者：
Krasikova A
影响因子：
3.4
作者：
Christiansen DG
通讯作者：
Christiansen DG