Cytological maps of lampbrush chromosomes of European water frogs (Pelophylax esculentus complex) from the Eastern Ukraine.
Cytological maps of lampbrush chromosomes of European water frogs (Pelophylax esculentus complex) from the Eastern Ukraine.
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DOI：
10.1186/1471-2156-14-26
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发表时间：
2013-04-16
期刊：
影响因子：
2.9
通讯作者：
Krasikova A
中科院分区：
文献类型：
--
作者：
Dedukh D;Mazepa G;Shabanov D;Rosanov J;Litvinchuk S;Borkin L;Saifitdinova A;Krasikova A
杂种发生（半克隆遗传）是亲本种之间通过与亲本种中的一种杂交繁殖出杂种的一种无性生殖。欧洲水蛙（Pelophylax esculentus complex）是研究种间杂交、半克隆遗传和多倍体化过程的合适模型。斑蛙（P. esculentus）复合体由湖蛙（P. ridibundus）和池蛙（P. lessonae）两个亲本种及其杂交后代斑蛙（P. esculentus）组成。亲本蛙和杂交蛙都能进行整体繁殖，形成半无性种群系统。为了研究水蛙种群系统维持的机制，需要对亲本和不同雌雄杂交动物配子中传递的核型进行表征。为了获得一种鉴定杂交雌蛙卵母细胞核型的工具，我们从原产于乌克兰东部的两个亲本种的卵母细胞中构建了灯刷染色体的细胞学图谱。我们进一步鉴定了染色体标记结构的某些分子成分，并绘制了富含卷曲蛋白的球体和颗粒、染色体相关核仁和积累剪接因子的特殊环。结果表明，在同源染色体长度、标记结构和间质重复位点（TTAGGG）的数量和位置以及核仁组织器活性等方面，柽柽树和小油桐的灯管染色体存在差异。在两种植物的灯刷染色体的着丝粒区定位到卫星重复RrS1。此外，我们在P. ridibundus和P. lessonae的卵母细胞中发现了RrS1重复转录本。此外，还发现了富含g的端粒重复序列转录本与黄颡鱼和黄颡鱼灯刷染色体末端区有关。本文构建的灯刷染色体细胞学图谱，为确定不同倍性、不同种群体系的青松雌虫卵母细胞内的基因组传播类型提供了依据。
Hybridogenesis (hemiclonal inheritance) is a kind of clonal reproduction in which hybrids between parental species are reproduced by crossing with one of the parental species. European water frogs (Pelophylax esculentus complex) represent an appropriate model for studying interspecies hybridization, processes of hemiclonal inheritance and polyploidization. P. esculentus complex consists of two parental species, P. ridibundus (the lake frog) and P. lessonae (the pool frog), and their hybridogenetic hybrid – P. esculentus (the edible frog). Parental and hybrid frogs can reproduce syntopically and form hemiclonal population systems. For studying mechanisms underlying the maintenance of water frog population systems it is required to characterize the karyotypes transmitted in gametes of parental and different hybrid animals of both sexes. In order to obtain an instrument for characterization of oocyte karyotypes in hybrid female frogs, we constructed cytological maps of lampbrush chromosomes from oocytes of both parental species originating in Eastern Ukraine. We further identified certain molecular components of chromosomal marker structures and mapped coilin-rich spheres and granules, chromosome associated nucleoli and special loops accumulating splicing factors. We recorded the dissimilarities between P. ridibundus and P. lessonae lampbrush chromosomes in the length of orthologous chromosomes, number and location of marker structures and interstitial (TTAGGG)n-repeat sites as well as activity of nucleolus organizer. Satellite repeat RrS1 was mapped in centromere regions of lampbrush chromosomes of the both species. Additionally, we discovered transcripts of RrS1 repeat in oocytes of P. ridibundus and P. lessonae. Moreover, G-rich transcripts of telomere repeat were revealed in association with terminal regions of P. ridibundus and P. lessonae lampbrush chromosomes. The constructed cytological maps of lampbrush chromosomes of P. ridibundus and P. lessonae provide basis to define the type of genome transmitted within individual oocytes of P. esculentus females with different ploidy and from various population systems.
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