Having a blast with gene transfer
Having a blast with gene transfer
复制标题
享受基因转移带来的乐趣
DOI：
10.1016/0960-9822(92)90421-6
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发表时间：
1992
期刊：
影响因子：
9.2
通讯作者：
D. Lo
中科院分区：
文献类型：
--
作者：
L. Pecorino;D. Lo
The ability to introduce cloned genes into cells, generally referred to as transformation or transfection; is one of the most powerful and far-reaching methodologies to come out of~molecular biology. It has played a critical role in the study of gene expression and protein stucture and function. However, many standard techniques work on only limited ranges of host cells and others are labor intensive or require large numbers of cells. Enter ‘biolistics’ (biological ballistics), a relatively new method of transfection that literally shoots high velocity microprojectiles, carrying DNA, into cells. It promises to be an efficient transfection method with much broader applicability. The biolistic process (for review see [1,2 3 > was originally developed by Sanford et al. [3] as a means of introducing DNA into plant cells. The limitations of existing methods of gene transfer stimulated the idea of shooting tungsten or gold particles coated with DNA directly into cells. Since then its use in transfection has extended well beyond plants to an ever-growing list of cell types, some of which had previously been recalcitrant to more routine methods of gene transfer.
影响因子：
56.9
作者：
JOHNSTON, SA;ANZIANO, PQ;BUTOW, RA
通讯作者：
BUTOW, RA
影响因子：
56.9
作者：
BOYNTON, JE;GILLHAM, NW;SANFORD, JC
通讯作者：
SANFORD, JC