Pathogenic and host range determinants of the feline aplastic anemia retrovirus.
Pathogenic and host range determinants of the feline aplastic anemia retrovirus.
复制标题
猫再生障碍性贫血逆转录病毒的致病性和宿主范围决定因素。
DOI：
10.1073/pnas.85.8.2758
复制
发表时间：
1988
影响因子：
11.1
通讯作者：
Mullins,JI
中科院分区：
文献类型：
--
作者：
Riedel,N;Hoover,EA;Dornsife,RE;Mullins,JI
猫白血病病毒（FeLV）C-Sarma（或FSC）是C亚群FeLV的原型，可在远交无特定病原体（SPF）猫中诱导致死性再生障碍性贫血。FeLV C分离株在体外还具有更广泛的宿主范围，包括在FeLV中独特的在豚鼠细胞中复制的能力。为了鉴定负责FSC致病性和宿主范围的病毒决定因子，我们通过在FSC和FeLV-61 E（或F6 A）之间交换基因片段构建了一系列前病毒DNA，后者的致病性最低，其体外宿主范围仅限于猫细胞。将包含pol（整合酶/核酸内切酶基因）3'端73个氨基酸密码子和env（细胞外糖蛋白（gp 70）基因）N端241个氨基酸密码子的FSC 886个碱基对（bp）片段转移到F6 A基因组中，足以使嵌合病毒具有诱导SPF猫致死性再生障碍性贫血的能力。与此相反，没有嵌合体缺乏这个序列诱导疾病。当在体外测定时，所有含有FSC的886-bp片段的嵌合病毒获得在异源细胞（包括狗和豚鼠细胞）中复制的能力。因此，猫再生障碍性贫血逆转录病毒的致病性和宿主范围决定子共定位于FSC基因组的3 ′ pol-5 ′ env区，并可能位于编码胞外糖蛋白N末端241个氨基酸残基的区域内。
Feline leukemia virus (FeLV) C-Sarma (or FSC) is a prototype of subgroup C FeLVs, which induce fatal aplastic anemia in outbred specific-pathogen-free (SPF) cats. FeLV C isolates also possess an extended host range in vitro, including an ability, unique among FeLVs, to replicate in guinea pig cells. To identify the viral determinants responsible for the pathogenicity and host range of FSC we constructed a series of proviral DNAs by exchanging gene fragments between FSC and FeLV-61E (or F6A), the latter of which is minimally pathogenic and whose host range in vitro is restricted to feline cells. Transfer of an 886-base-pair (bp) fragment of FSC, encompassing the codons for 73 amino acids at the 3' end of pol (the integrase/endonuclease gene) and the codons for 241 amino acids of the N-terminal portion of env [the extracellular glycoprotein (gp70) gene], into the F6A genome was sufficient to confer onto chimeric viruses the ability to induce fatal aplastic anemia in SPF cats. In contrast, no chimera lacking this sequence induced disease. When assayed in vitro, all chimeric viruses containing the 886-bp fragment of FSC acquired the ability to replicate in heterologous cells, including dog and guinea pig cells. Thus, the pathogenic and the host range determinants of the feline aplastic anemia retrovirus colocalize to a 3' pol-5' env region of the FSC genome and likely reside within a region encoding 241 amino acid residues of the N terminus of the extracellular glycoprotein.
自然发生的猫白血病中 c-myc 基因的病毒转导
影响因子：
64.8
作者：
J. Mullins;David S. Brody;R. Binari;S. Cotter
通讯作者：
S. Cotter