Viral transduction of c-myc gene in naturally occurring feline leukaemias
Viral transduction of c-myc gene in naturally occurring feline leukaemias
复制标题
自然发生的猫白血病中 c-myc 基因的病毒转导
作者：
J. Mullins;David S. Brody;R. Binari;S. Cotter
猫白血病病毒（FeLV）在流行病学上与家猫的大多数淋巴肿瘤诱导相关1。然而，这些肿瘤中约有三分之一缺乏外源性病毒或仅在肿瘤生长后显示病毒整合的证据1，2。为了帮助确定猫淋巴瘤发生的遗传机制，我们在这里探讨了细胞癌基因（c-onc基因）在肿瘤细胞DNA中重排的可能性。在31例自然发生的淋巴瘤中的16例FeLV阳性T细胞肿瘤中，2例显示出含有myc癌基因序列的重组FeLV前病毒的证据。其中一个产生了可传播的含myc的FeLV。在这两种情况下，c-myc及其周围的DNA似乎没有改变。我们认为，myc与FeLV的关联可能导致其激活，并在猫T细胞淋巴瘤的重要部分的发展中发挥作用。我们的发现与实验诱导鸡淋巴瘤的研究相反，在鸡淋巴瘤中，myc的激活是通过在c-myc附近插入逆转录病毒启动子而不是通过掺入病毒来发生的（参考文献3-5）。
Feline leukaemia virus (FeLV) is epidemiologically associated with induction of the majority of lymphoid tumours of the domestic cat1. However, about one-third of these tumours are devoid of exogenous virus or show evidence of virus integration only after tumour outgrowth1,2. To help define the genetic mechanisms of feline lymphomagenesis we have explored here the possibility that cellular oncogenes (c-onc genes) are rearranged in tumour cell DNA. Of 16 FeLV-positive T-cell tumours among 31 naturally occurring lymphomas, 2 showed evidence of recombinant FeLV proviruses containing myc oncogene sequences. One of the two produced a transmissible myc-containing FeLV. In both cases c-myc and its surrounding DNA appeared unaltered. We believe that the association of myc with FeLV may result in its activation and play a part in the development of a significant fraction of cat T-cell lymphomas. Our findings contrast with studies of experimental induction of chicken lymphoma, in which myc activation occurs by retrovirus promoter insertion near c-myc (refs 3–5), rather than by incorporation into virus.
通过体内重组和选择从噬菌体文库中纯化基因组序列。
影响因子：
14.9
作者：
Seed,B
通讯作者：
Seed,B
受感染细胞中猫白血病病毒 DNA 的序列组织。
影响因子：
14.9
作者：
Mullins,JI;Casey,JW;Nicolson,MO;Davidson,N
通讯作者：
Davidson,N