Role of Toll-like receptor (TLR) 2 in experimental Bacillus cereus endophthalmitis.
Role of Toll-like receptor (TLR) 2 in experimental Bacillus cereus endophthalmitis.
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DOI：
10.1371/journal.pone.0028619
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Callegan MC
中科院分区：
文献类型：
--
作者：
Novosad BD;Astley RA;Callegan MC
蜡样芽孢杆菌引起一种独特的快速和致盲的眼内感染，眼内炎。B。蜡样芽胞杆菌在眼睛中复制，合成大量毒素，并引发爆炸性眼内炎症。涉及快速和爆炸性眼内免疫反应的机制尚未得到解决。由于Toll样受体（TLR）是感染过程中生物体初始识别的组成部分，我们假设在B.蜡状菌眼内炎直接受到TLR2（一种已知的革兰氏阳性病原体识别受体）的存在的影响。为了解决这个假设，我们比较了实验B的过程。在野生型C57BL/6J小鼠中，蜡样眼球内炎的发生率与年龄匹配的纯合TLR 2-/-小鼠的发生率相比。输出参数包括细菌生长、炎性细胞（PMN）浸润、细胞因子/趋化因子动力学、视网膜功能检测和组织学分析，N ≥ 4只眼/试验/时间点/小鼠品系。B。无论小鼠品系如何，蜡样芽胞杆菌在12小时内以相似的速度生长至108 CFU/眼。与感染的野生型眼睛相比，在感染的TLR2-/-眼睛中视网膜功能在更大程度上得到保留，但是两种小鼠品系的感染的眼睛丧失了显著的功能。与感染的野生型眼睛相比，在感染的TLR 2-/-眼睛中保留了视网膜结构，具有有限的视网膜和玻璃体细胞浸润。眼部髓过氧化物酶活性证实了这些结果。一般而言，在感染的野生型眼睛中检测到的TNF α、IFN γ、IL 6和KC浓度高于感染的TLR 2-/-眼睛。TLR2的缺乏导致眼内促炎细胞因子/趋化因子水平降低和炎性细胞向眼内的募集改变，导致眼内炎症减少和视网膜结构的保留，以及视网膜功能的程度略高。这些结果表明TLR 2是对B的初始眼部反应的重要组分。蜡样眼内炎
Bacillus cereus causes a uniquely rapid and blinding intraocular infection, endophthalmitis. B. cereus replicates in the eye, synthesizes numerous toxins, and incites explosive intraocular inflammation. The mechanisms involved in the rapid and explosive intraocular immune response have not been addressed. Because Toll-like receptors (TLRs) are integral to the initial recognition of organisms during infection, we hypothesized that the uniquely explosive immune response observed during B. cereus endophthalmitis is directly influenced by the presence of TLR2, a known Gram-positive pathogen recognition receptor. To address this hypothesis, we compared the courses of experimental B. cereus endophthalmitis in wild type C57BL/6J mice to that of age-matched homozygous TLR2-/- mice. Output parameters included analysis of bacterial growth, inflammatory cell (PMN) infiltration, cytokine/chemokine kinetics, retinal function testing, and histology, with N≥4 eyes/assay/time point/mouse strain. B. cereus grew at similar rates to108 CFU/eye by 12 h, regardless of the mouse strain. Retinal function was preserved to a greater degree in infected TLR2-/- eyes compared to that of infected wild type eyes, but infected eyes of both mouse strains lost significant function. Retinal architecture was preserved in infected TLR2-/- eyes, with limited retinal and vitreal cellular infiltration compared to that of infected wild type eyes. Ocular myeloperoxidase activities corroborated these results. In general, TNFα, IFNγ, IL6, and KC were detected in greater concentrations in infected wild type eyes than in infected TLR2-/- eyes. The absence of TLR2 resulted in decreased intraocular proinflammatory cytokine/chemokine levels and altered recruitment of inflammatory cells into the eye, resulting in less intraocular inflammation and preservation of retinal architecture, and a slightly greater degree of retinal function. These results demonstrate TLR2 is an important component of the initial ocular response to B. cereus endophthalmitis.
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影响因子：
4.1
作者：
DAVID, DB;KIRKBY, GR;NOBLE, BA
通讯作者：
NOBLE, BA
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影响因子：
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作者：
Park, JM;Ng, VH;Maeda, S;Rest, RE;Karin, M
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3.1
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