RNAi in Arthropods: Insight into the Machinery and Applications for Understanding the Pathogen-Vector Interface.
RNAi in Arthropods: Insight into the Machinery and Applications for Understanding the Pathogen-Vector Interface.
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DOI：
10.3390/genes3040702
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发表时间：
2012-11-06
期刊：
影响因子：
3.5
通讯作者：
Maritz-Olivier C
中科院分区：
文献类型：
--
作者：
Barnard AC;Nijhof AM;Fick W;Stutzer C;Maritz-Olivier C
基因组测序数据的可用性与通过转录组和蛋白质组数据了解表达基因的知识相结合，极大地增进了我们对节肢动物疾病载体的理解。我们不仅深入了解了媒介生物学，而且了解了它们各自的媒介-病原体相互作用。通过结合后基因组数据库和 RNAi 等反向遗传方法的优势，可用药物和疫苗靶标的数量以及后续转基因或副转基因方法的转基因数量都得到了扩大。这些现在为病媒及其病原体的现场控制策略铺平了道路。基本的科学问题，例如了解载体 RNAi 机器的基本组件，至关重要，因为这可以将基本的 RNAi 机器组件转移到 RNAi 缺陷的载体中，从而扩展这些 RNAi 缺陷的载体和病原体的遗传工具箱。在这篇综述中，我们重点关注节肢动物载体 RNAi 机制的当前知识，以及 RNAi 对理解载体生物学和载体-病原体相互作用的影响，载体基因组数据可在 VectorBase 上获得。
The availability of genome sequencing data in combination with knowledge of expressed genes via transcriptome and proteome data has greatly advanced our understanding of arthropod vectors of disease. Not only have we gained insight into vector biology, but also into their respective vector-pathogen interactions. By combining the strengths of postgenomic databases and reverse genetic approaches such as RNAi, the numbers of available drug and vaccine targets, as well as number of transgenes for subsequent transgenic or paratransgenic approaches, have expanded. These are now paving the way for in-field control strategies of vectors and their pathogens. Basic scientific questions, such as understanding the basic components of the vector RNAi machinery, is vital, as this allows for the transfer of basic RNAi machinery components into RNAi-deficient vectors, thereby expanding the genetic toolbox of these RNAi-deficient vectors and pathogens. In this review, we focus on the current knowledge of arthropod vector RNAi machinery and the impact of RNAi on understanding vector biology and vector-pathogen interactions for which vector genomic data is available on VectorBase.
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影响因子：
4
作者：
Araujo, R. N.;Campos, I. T. N.;Pereira, M. H.
通讯作者：
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影响因子：
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DOI：
10.1073/pnas.0502815102
发表时间：
2005-09-20
影响因子：
11.1
作者：
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通讯作者：
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影响因子：
3.8
作者：
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