Cyclic stretch regulates immune responses via tank-binding kinase 1 expression in macrophages.
Cyclic stretch regulates immune responses via tank-binding kinase 1 expression in macrophages.
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DOI：
10.1002/2211-5463.13526
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发表时间：
2023-01
期刊：
影响因子：
2.6
通讯作者：
Oishi, Yumiko
中科院分区：
文献类型：
--
作者：
Nakagawa, Anna;Hayakawa, Sumio;Cheng, Yinglan;Honda, Azusa;Yuzawa, Ryo;Ogawa, Rei;Oishi, Yumiko
分布在全身组织中的巨噬细胞有助于体内平衡。在炎症状态下，巨噬细胞经历调节免疫应答和各种细胞功能的信号转导的机械应力。然而，在生理周期性拉伸下炎症反应对巨噬细胞的影响尚不清楚。我们发现生理性周期性牵张通过调节NF-κB活性抑制巨噬细胞中炎性细胞因子的表达。在巨噬细胞中，NF-κB在Ser 536处的磷酸化受到抑制，表明罐结合激酶（TBK 1）在生理应激期间调节NF-κB活性。此外，TBK 1表达被生理牵拉抑制，并且通过siRNA敲低TBK 1诱导NF-κB在Ser 536处磷酸化的抑制。总之，生理拉伸触发了TBK 1依赖性过度炎症反应的抑制，这可能是维持组织稳态所必需的。分布在全身组织中的巨噬细胞有助于体内平衡。我们发现生理性周期性牵张通过调节NF-κB活性来抑制炎性细胞因子的表达。此外，TBK 1也被抑制，TBK 1是连接受体与其下游基因表达的衔接分子。生理拉伸触发TBK 1依赖性过度炎症反应的抑制，这可能是维持组织稳态所必需的。
Macrophages distributed in tissues throughout the body contribute to homeostasis. In the inflammatory state, macrophages undergo mechanical stress that regulates the signal transduction of immune responses and various cellular functions. However, the effects of the inflammatory response on macrophages under physiological cyclic stretch are unclear. We found that physiological cyclic stretch suppresses inflammatory cytokine expression in macrophages by regulating NF‐κB activity. NF‐κB phosphorylation at Ser536 in macrophages was inhibited, suggesting that tank‐binding kinase (TBK1) regulates NF‐κB activity during physiological stress. Moreover, TBK1 expression was suppressed by physiological stretch, and TBK1 knockdown by siRNA induced the suppression of NF‐κB phosphorylation at Ser536. In conclusion, physiological stretch triggers suppression of a TBK1‐dependent excessive inflammatory response, which may be necessary to maintain tissue homeostasis. Macrophages distributed in tissues throughout the body contribute to homeostasis. We found that physiological cyclic stretch suppresses inflammatory cytokine expression by regulating NF‐κB activity. Moreover, TBK1, which is an adaptor molecule linking receptors to its downstream gene expression, was also suppressed. Physiological stretch triggers suppression of a TBK1‐dependent excessive inflammatory response, which may be necessary to maintain tissue homeostasis.
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影响因子：
6
作者：
Christian F;Smith EL;Carmody RJ
通讯作者：
Carmody RJ
影响因子：
64.5
作者：
Lavin Y;Winter D;Blecher-Gonen R;David E;Keren-Shaul H;Merad M;Jung S;Amit I
通讯作者：
Amit I
影响因子：
4.8
作者：
Buss, H;Dörrie, A;Kracht, M
通讯作者：
Kracht, M
DOI：
10.1165/rcmb.2018-0122oc
发表时间：
2019-03-01
影响因子：
6.4
作者：
Hagan, Robert S.;Torres-Castillo, Jose;Doerschuk, Claire M.
通讯作者：
Doerschuk, Claire M.
影响因子：
3.5
作者：
Kanjanamekanant, K.;Luckprom, P.;Pavasant, P.
通讯作者：
Pavasant, P.