Heavy labeling of recombinant proteins.
Heavy labeling of recombinant proteins.
复制标题
重组蛋白的重标记。
DOI：
10.1007/978-1-59745-456-8_11
复制
发表时间：
2007
期刊：
影响因子：
--
通讯作者：
Rodriguez,Eric
中科院分区：
文献类型：
--
作者：
Rodriguez,Eric
Because of the cost of isotopic chemicals and heterologous proteins to produce, an economical15N/13C isotopic labeling method is critically needed. Four protocols have been tested for the expression of Ovine interferon-τ inPichia pastoris.13C-glucose in place of13C-glycerol as well as the need for15N/13C-sources were evaluated during the growth phase. Sequential addition of15NH4Cl and13C-methanol were also evaluated at different ratio. Our results demonstrate that15N/13C isotopes are not required throughout the initial growth period but are necessary at low concentration a few hours prior to the methanol induction period. We have evaluated the cost of the use of isotopes15NH4Cl,13C-glucose and13C-methanol in our optimised P4 protocol conditions. The cost was one-third that of the standard method using15NH4Cl and13C-glucose throughout the entire growth period and was even lower using13C-glycerol.
影响因子：
11.2
作者：
C. Pontzer;F. Bazer;Howard M. Johnson
通讯作者：
C. Pontzer;F. Bazer;Howard M. Johnson
甲基营养酵母毕赤酵母中表达的蜱抗凝血肽 (TAP) 的高水平分泌和非常有效的同位素标记
DOI：
--
发表时间：
1994
期刊：
Bio/Technology
影响因子：
--
作者：
Y. Laroche;V. Storme;J. Meutter;J. Messens;M. Lauwereys
通讯作者：
M. Lauwereys
DOI：
10.1016/s0006-291x(88)80109-8
发表时间：
1988-04-29
影响因子：
3.1
作者：
PONTZER, CH;TORRES, BA;JOHNSON, HM
通讯作者：
JOHNSON, HM