Application of microfluidic chip technology to study the inhibitory effect of tetramethylpyrazine on platelet aggregation, activation, and phosphatidylserine exposure mediated by pathological high shear rate
Application of microfluidic chip technology to study the inhibitory effect of tetramethylpyrazine on platelet aggregation, activation, and phosphatidylserine exposure mediated by pathological high shear rate
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应用微流控芯片技术研究四甲基吡嗪对病理性高剪切速率介导的血小板聚集、活化及磷脂酰丝氨酸暴露的抑制作用
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发表时间：
2022
影响因子：
1.1
通讯作者：
Yuan Li
中科院分区：
文献类型：
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作者：
Tian Zhang;Ling Liu;Xiaojing Huang;Xuemei Gao;Dan Chen;Xuanrong Huan;Cui He;Yuan Li
目的研究川芎嗪（TMA）的抗血栓作用及其机制。方法本研究建立了模拟正常动脉和狭窄动脉的微流控芯片模型，研究了TMA对血小板聚集、活化（P-选择素、GPIIb/IIIa、单核细胞-血小板聚集体）和磷脂酰丝氨酸（PS）暴露的抑制作用。此外，我们还用比浊法研究了TMA对ADP和瑞斯托霉素诱导的血小板聚集的影响。结果TMA对病理性高切变率诱导的血小板聚集、活化及PS暴露有明显的抑制作用。在静态条件下，TMA可抑制ADP和瑞斯托霉素诱导的血小板聚集。结论TMA主要通过抑制血管性血友病因子（vWF）与GPIb/IX/V复合物的结合而抑制血小板聚集、活化和PS暴露，并通过血小板P2 Y12-ADP受体途径部分抑制血小板聚集。
Objective In order to study the antithrombotic effect and mechanism of tetramethylpyrazine (TMA). Methods In this study, we developed a microfluidic chip model that can mimic normal arteries and stenotic arterial vessels, and studied the inhibitory effects of TMA on platelet aggregation, activation (P-selectin, GPIIb/IIIa, monocyte-platelet aggregates) and phosphatidyl serine (PS) exposure. In addition, we also investigated the effect of TMA on ADP and ristocetin-induced platelet aggregation by turbidimetry. Results The results showed that TMA significantly inhibited the platelet aggregation, activation and PS exposure induced by pathological high shear rate. Under static conditions, TMA can inhibit ADP and ristocetin-induced platelet aggregation. Conclusion The results indicated that TMA mainly inhibited platelet aggregation, activation and PS exposure by inhibiting the binding of von Willebrand factor (vWF) to the GPIb/IX/V complex, and partially inhibited platelet aggregation through the platelet P2Y12–ADP receptor pathway.
影响因子：
20.3
作者：
S. Hamburger;R. McEver
通讯作者：
S. Hamburger;R. McEver
影响因子：
3.4
作者：
Feghhi, Shirin;Munday, Adam D.;Sniadecki, Nathan J.
通讯作者：
Sniadecki, Nathan J.
抗血小板药物抑制剪切诱导的血小板粘附和活化的微流体测定。
影响因子：
6.1
作者：
Rahman,ShekhMojibur;Hlady,Vladimir
通讯作者：
Hlady,Vladimir