Ionizing radiation-induced bystander mutagenesis and adaptation: quantitative and temporal aspects.
Ionizing radiation-induced bystander mutagenesis and adaptation: quantitative and temporal aspects.
复制标题
DOI：
10.1016/j.mrfmmm.2009.08.006
复制
发表时间：
2009-12-01
影响因子：
2.3
通讯作者：
Liber, Howard L.
中科院分区：
文献类型：
--
作者：
Zhang, Ying;Zhou, Junqing;Baldwin, Joseph;Held, Kathryn D.;Prise, Kevin M.;Redmond, Robert W.;Liber, Howard L.
这项工作探讨了几个定量方面的辐射诱导的旁观者诱变WTK 1人淋巴母细胞。使用细胞的γ照射来产生含有旁观者信号的条件培养基，并将该培养基转移到幼稚受体细胞上。动力学研究表明，它需要长达一个小时，以产生足够的信号，以诱导最大水平的突变的胸苷激酶位点在旁观者细胞接受条件培养基。此外，它需要暴露于旁观者细胞中的信号至少一个小时才能诱导突变。旁观者信号在培养基中相当稳定，需要12-24小时才能消失。含有旁观者信号的培养基通过4倍稀释而变得无效;相反，需要照射以产生旁观者信号的细胞数量减少20倍以上以消除旁观者诱导的诱变。这表明旁观者信号的某种反馈抑制阻止了信号细胞释放更多的信号。最后，电离辐射诱导的适应性反应被证明在减少旁观者诱变方面是有效的;此外，在转移的培养基中低水平暴露于旁观者信号诱导的适应性在减少随后的γ射线暴露诱导的突变方面是有效的。
This work explores several quantitative aspects of radiation-induced bystander mutagenesis in WTK1 human lymphoblast cells. Gamma-irradiation of cells was used to generate conditioned medium containing bystander signals, and that medium was transferred onto naïve recipient cells. Kinetic studies revealed that it required up to one hour to generate sufficient signal to induce the maximal level of mutations at the thymidine kinase locus in the bystander cells receiving the conditioned medium. Furthermore, it required at least one hour of exposure to the signal in the bystander cells to induce mutations. Bystander signal was fairly stable in the medium, requiring 12–24 hours to diminish. Medium that contained bystander signal was rendered ineffective by a 4-fold dilution; in contrast a greater than 20-fold decrease in the cell number irradiated to generate a bystander signal was needed to eliminate bystander-induced mutagenesis. This suggested some sort of feedback inhibition by bystander signal that prevented the signaling cells from releasing more signal. Finally, an ionizing radiation-induced adaptive response was shown to be effective in reducing bystander mutagenesis; in addition, low levels of exposure to bystander signal in the transferred medium induced adaptation that was effective in reducing mutations induced by subsequent γ-ray exposures.
登录
查看更多内容
影响因子：
3.4
作者：
Coleman, MA;Yin, E;Wyrobek, AJ
通讯作者：
Wyrobek, AJ
DOI：
10.1016/0165-7992(93)90036-u
发表时间：
1993-02-01
期刊：
MUTATION RESEARCH
影响因子：
--
作者：
DOMINGUEZ, I;PANNEERSELVAM, N;CORTES, F
通讯作者：
CORTES, F
影响因子：
8.8
作者：
Chen, S.;Zhao, Y.;Han, W.;Zhao, G.;Zhu, L.;Wang, J.;Bao, L.;Jiang, E.;Xu, A.;Hei, T. K.;Yu, Z.;Wu, L.
通讯作者：
Wu, L.
影响因子：
2.6
作者：
Mitchell, SA;Marino, SA;Hall, EJ
通讯作者：
Hall, EJ
影响因子：
8
作者：
Burdak-Rothkamm, S.;Short, S. C.;Prise, K. M.
通讯作者：
Prise, K. M.