Primary structure of the immunoglobulin J chain from the mouse.
Primary structure of the immunoglobulin J chain from the mouse.
复制标题
小鼠免疫球蛋白 J 链的一级结构。
DOI：
10.1073/pnas.79.21.6656
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发表时间：
1982
影响因子：
11.1
通讯作者：
Koshland,ME
中科院分区：
文献类型：
--
作者：
Cann,GM;Zaritsky,A;Koshland,ME
通过对来自两个独立克隆的嵌合质粒的J链cDNA插入片段进行序列分析，研究了小鼠J链的一级结构。序列分析表明，（i）两个cDNA插入片段占据了J链mRNA的所有5'端核苷酸，但约有100个;（ii）J链mRNA编码至少23个氨基酸的前肽、137个残基的成熟蛋白和707个核苷酸的3'端非翻译区（不包括3'端poly（A）区）。成熟小鼠J链推导的氨基酸序列被认为是74%相同的人J链与先前确定的。通过分析序列的保守特征，产生了一个双结构域结构的J链，这与其在IgM和伊加聚合中的功能密切相关。此外，通过比较小鼠和人的J和重链的同源性，获得了参与聚合的结构是免疫球蛋白分子中最保守的元件的证据。
The primary structure of the murine J chain was investigated by sequence analysis of the J chain cDNA inserts from two independently cloned chimeric plasmids. The sequence data showed that (i) the two cDNA inserts accounted for all but approximately 100 5' nucleotides of the J chain mRNA and (ii) the J chain mRNA encodes a prepeptide of at least 23 amino acids, a mature protein of 137 residues, and an untranslated 3' region of 707 nucleotides exclusive of the 3' poly(A) tract. The amino acid sequence deduced for the mature mouse J chain was found to be 74% identical with that previously determined for the human J chain. By analyzing the conserved features of the sequence, a two-domain structure was generated for the J chain which correlates well with its functions in the polymerization of IgM and IgA. Moreover, by comparing the homologies of the J and heavy chains in mouse and man, evidence was obtained that the structures involved in polymerization are the most conserved elements of immunoglobulin molecules.
免疫化学
影响因子：
11.4
作者：
H. M. Grey;R. A. Phillips;A. Rosenthal;Darcy B. Wilson
通讯作者：
Darcy B. Wilson
免疫球蛋白系统
DOI：
10.1007/978-1-4613-4550-3
发表时间：
1974
期刊：
Developments in biological standardization
影响因子：
--
作者：
J. Mestecky;A. Lawton
通讯作者：
A. Lawton