Noninvasive assessment of radiation-induced renal injury in mice.
Noninvasive assessment of radiation-induced renal injury in mice.
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DOI：
10.1080/09553002.2021.1876950
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发表时间：
2021
影响因子：
2.6
通讯作者：
Marples B
中科院分区：
文献类型：
--
作者：
Ahmad A;Shi J;Ansari S;Afaghani J;Molina J;Pollack A;Merscher S;Zeidan YH;Fornoni A;Marples B
肾脏是一种对辐射敏感的晚反应正常组织。其损伤的特征为放射性肾病和肾小球滤过率（GFR）下降。本研究旨在将两种快速且经济高效的评估GFR的方法与更为传统的生物标志物测量方法进行比较。
对C57BL/6小鼠进行双侧局部X射线照射（单次14Gy或5次，每次6Gy）。在治疗20周后评估肾脏损伤的功能指标。通过经皮测量异硫氰酸荧光素共轭（FITC）-右旋糖酐肾排泄以及使用造影剂（碘帕醇300，ISOVUE - 300）的动态对比增强CT成像来估算GFR。
苏木精 - 伊红（H&E）和过碘酸雪夫（PAS）染色分别显示，两种照射方案后均出现了相当程度的辐射诱导的肾小球萎缩和系膜基质积聚，尽管分次照射方案导致的弥漫性肾小管间质纤维化程度较轻。照射后白蛋白与肌酐比值升高（单次14Gy：100.4±12.2μg/mg；5次，每次6Gy：80.4±3.02μg/mg），是未治疗对照组（44.9±3.64μg/mg）的两倍。两种技术所定义的GFR与血尿素氮、系膜扩张评分和血清肌酐呈负相关。FITC - 右旋糖酐经皮测量方法更为快速，可用于评估清醒动物的GFR，动态对比增强CT成像技术同样安全有效。
本研究表明，与经皮测量方法相比，通过动态对比增强CT成像测量GFR在评估肾功能方面安全有效。
The kidney is a radiosensitive late-responding normal tissue. Injury is characterized by radiation nephropathy and decline of glomerular filtration rate (GFR). The current study aimed to compare two rapid and cost-effective methodologies of assessing GFR against more conventional biomarker measurements. C57BL/6 mice were treated with bilateral focal X-irradiation (1x14Gy or 5x6Gy). Functional measurements of kidney injury were assessed 20 weeks post-treatment. GFR was estimated using a transcutaneous measurement of Fluorescein-isothiocyanate conjugated (FITC)-sinistrin renal excretion and also dynamic contrast-enhanced CT imaging with a contrast agent (ISOVUE-300 Iopamidol). H&E and PAS staining identified comparable radiation-induced glomerular atrophy and mesangial matrix accumulation after both radiation schedules respectively, although the fractionated regimen resulted in less diffuse tubulointerstitial fibrosis. Albumin-to-creatinine ratios increased after irradiation (1x14Gy: 100.4±12.2μg/mg; 6x5Gy: 80.4±3.02 μg/mg) and were double that of non-treated controls (44.9±3.64 μg/mg). GFR defined by both techniques was negatively correlated with BUN, mesangial expansion score and serum creatinine. The FITC-sinistrin transcutaneous method was more rapid and can be used to assess GFR in conscious animals, dynamic contrast-enhanced CT imaging technique was equally safe and effective. This study demonstrated that GFR measured by dynamic contrast-enhanced CT imaging is safe and effective compared to transcutaneous methodology to estimate kidney function.
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影响因子：
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