Evaluation of the interactions of HIV-1 integrase with small ubiquitin-like modifiers and their conjugation enzyme Ubc9.
Evaluation of the interactions of HIV-1 integrase with small ubiquitin-like modifiers and their conjugation enzyme Ubc9.
复制标题
评估 HIV-1 整合酶与小泛素样修饰物及其缀合酶 Ubc9 的相互作用。
DOI：
10.3892/ijmm.2012.1088
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发表时间：
2012-11
期刊：
影响因子：
--
通讯作者：
陈金中
中科院分区：
文献类型：
--
作者：
陈金中
Human immunodeficiency virus type 1 (HIV-1) integrase mediates the integration of reverse-transcribed viral cDNA into the genome of the host for the stable maintenance of the viral genome and the persistence of HIV-1 infection. In this study, the relationships between HIV-1 integrase (HIV-1 IN) and three SUMO conjugation pathway proteins, as well as the effects of these associations, were investigated. The overexpression of SUMO1/SUMO2 and Ubc9 changed the intracellular localization of HIV-1 IN from a diffuse distribution to a punctate localization. SUMO1, SUMO2 and Ubc9 were shown to interact with HIV-1 IN. The SUMOylation of HIV-1 IN was verified. In addition, SUMO1, SUMO2 and Ubc9 were shown to influence the integration of both lentivirus and HIV-1. The overexpression of Ubc9 inhibited viral genome integration, and the upregulation of SUMO1 or SUMO2 enhanced the inhibitory effect of Ubc9. Knockdown of the endogenous levels of SUMO1, SUMO2 and Ubc9 increased the level of viral integration, while reverse transcription and the nuclear import of preintegration complex (PIC) were not affected. Our findings suggest that SUMO conjugation pathway proteins may act as cellular restriction factors and be detrimental to HIV-1 infection. These findings merit further investigation because of their potentially significant implications for the cellular antiviral response to HIV-1 infection.
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影响因子：
3.9
作者：
Wen-Zhi Guo;S. Sugaya;M. Satoh;T. Tomonaga;F. Nomura;T. Hiwasa;M. Takiguchi;K. Kita;N. Suzuki
通讯作者：
Wen-Zhi Guo;S. Sugaya;M. Satoh;T. Tomonaga;F. Nomura;T. Hiwasa;M. Takiguchi;K. Kita;N. Suzuki
DOI：
10.1016/b978-0-12-801946-7.00004-3
发表时间：
2015-07-24
期刊：
Principles of Molecular Virology
影响因子：
--
作者：
Cann AJ
通讯作者：
Cann AJ
影响因子：
30.3
作者：
Zhou, Honglin;Xu, Min;Espeseth, Amy S.
通讯作者：
Espeseth, Amy S.
DOI：
10.1007/s00018-007-7137-4
发表时间：
2007-12
期刊：
Cellular and molecular life sciences : CMLS
影响因子：
--
作者：
Zhao J
通讯作者：
Zhao J
影响因子：
13.8
作者：
B. Van Maele;K. Busschots;L. Vandekerckhove;F. Christ;Z. Debyser
通讯作者：
B. Van Maele;K. Busschots;L. Vandekerckhove;F. Christ;Z. Debyser