Dorsal horn administration of muscimol abolishes the muscle pressor reflex.
Dorsal horn administration of muscimol abolishes the muscle pressor reflex.
复制标题
背角给予蝇蕈醇可消除肌肉加压反射。
DOI：
--
复制
发表时间：
2001
影响因子：
3.3
通讯作者：
L. Wilson
中科院分区：
文献类型：
--
作者：
L. Wilson
本研究的目的是确定阻断背角突触传递对肌肉传入神经元激活所产生的心血管反应的影响。将GABA(A)激动剂muscimol应用于脊髓背表面可阻断突触传递。用-氯氯蔗糖和氨基甲酸乙酯麻醉猫，并进行椎板切除术。除L(7)背根外，从L(5)到S(2)的背根和腹根在一侧切开，电刺激L(7)和S(1)腹根的外周末端，引起同侧三头肌表面肌的静态收缩。脊髓L(4)- S(3)段的背表面被封闭在一个“井”中，该“井”是由乙烯基聚硅氧烷层形成的。将1mm的muscimol溶液（基于剂量反应数据）注入该井中，消除了反射性升压对收缩的反应（muscimol之前的平均动脉血压变化为47 +/- 7mmhg， muscimol之后的平均动脉血压变化为3 +/- 2mmhg）。肌肉拉伸使肌肉注射前平均动脉血压升高30 +/- 8 mmHg，而药物注射后平均动脉血压仅升高3 +/- 2 mmHg。将muscimol限制在L(7)节段会减弱加压剂对收缩（37 +/- 7至24 +/- 11 mmHg）和拉伸（28 +/- 2至16 +/- 8 mmHg）的反应。这些数据表明脊髓背角包含一个压迫反射的强制性突触。此外，这些数据支持了一种假设，即初级传入神经元的分支，而不是椎管内通路，负责压力反射的多节段整合。
The purpose of this study was to determine the effect of blocking synaptic transmission in the dorsal horn on the cardiovascular responses produced by activation of muscle afferent neurons. Synaptic transmission was blocked by applying the GABA(A) agonist muscimol to the dorsal surface of the spinal cord. Cats were anesthetized with alpha-chloralose and urethane, and a laminectomy was performed. With the exception of the L(7) dorsal root, the dorsal and ventral roots from L(5) to S(2) were sectioned on one side, and static contraction of the ipsilateral triceps surae muscle was evoked by electrically stimulating the peripheral ends of the L(7) and S(1) ventral roots. The dorsal surface of the L(4)--S(3) segments of the spinal cord were enclosed within a "well" created by applying layers of vinyl polysiloxane. Administration of a 1 mM solution of muscimol (based on dose-response data) into this well abolished the reflex pressor response to contraction (change in mean arterial blood pressure before was 47 +/- 7 mmHg and after muscimol was 3 +/- 2 mmHg). Muscle stretch increased mean arterial blood pressure by 30 +/- 8 mmHg before muscimol, but after drug application stretch increased MAP by only 3 +/- 2 mmHg. Limiting muscimol to the L(7) segment attenuated the pressor responses to contraction (37 +/- 7 to 24 +/- 11 mmHg) and stretch (28 +/- 2 to 16 +/- 8 mmHg). These data suggest that the dorsal horn of the spinal cord contains an obligatory synapse for the pressor reflex. Furthermore, these data support the hypothesis that branches of primary afferent neurons, not intraspinal pathways, are responsible for the multisegmental integration of the pressor reflex.
登录
查看更多内容
猫背角的 NMDA 受体阻断会减弱对肌肉收缩和伸展的反射性升压反应。
DOI：
10.1152/ajpheart.1996.270.2.h500
发表时间：
1996
期刊：
The American journal of physiology.
影响因子：
--
作者：
Hand,GA;Meintjes,AF;Keister,AW;Ally,A;Wilson,LB
通讯作者：
Wilson,LB
麻醉猫加压反射运动期间脊髓中 P 物质的释放。
DOI：
10.1113/jphysiol.1993.sp019460
发表时间：
1993
期刊：
The Journal of physiology
影响因子：
--
作者：
Wilson,LB;Fuchs,IE;Matsukawa,K;Mitchell,JH;Wall,PT
通讯作者：
Wall,PT
分级肌肉收缩对脊髓 P 物质释放、动脉血压和心率的影响。
影响因子：
20.1
作者：
Wilson,LB;Fuchs,IE;Mitchell,JH
通讯作者：
Mitchell,JH
鞘内阻断 NMDA 和非 NMDA 受体会减弱猫的运动升压反射。
DOI：
10.1152/jappl.1996.80.1.315
发表时间：
1996
期刊：
Journal of applied physiology (Bethesda, Md. : 1985)
影响因子：
--
作者：
Adreani,CM;Hill,JM;Kaufman,MP
通讯作者：
Kaufman,MP
静态肌肉收缩引起的升压反射：一氧化氮在背角的作用。
DOI：
10.1152/ajpregu.1999.276.6.r1639
发表时间：
1999
期刊：
The American journal of physiology.
影响因子：
--
作者：
Wilson,LB;Engbretson,J;Crews,AD
通讯作者：
Crews,AD