Substance P release in the spinal cord during the exercise pressor reflex in anaesthetized cats.
Substance P release in the spinal cord during the exercise pressor reflex in anaesthetized cats.
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麻醉猫加压反射运动期间脊髓中 P 物质的释放。
DOI：
10.1113/jphysiol.1993.sp019460
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
Wall,PT
中科院分区：
文献类型：
--
作者：
Wilson,LB;Fuchs,IE;Matsukawa,K;Mitchell,JH;Wall,PT
1.本研究的目的是确定静态骨骼肌收缩是否会导致脊髓L7-背角区域释放P物质（SP）。进行椎板切除术以暴露α-氯醛糖麻醉猫的脊髓。切断L 6脊神经根。将微透析探针插入L7背角区域，与收缩的小腿三头肌同侧。以3 μ l/min的缓冲液灌注探针。通过放射免疫测定法从微透析样品中测量P物质样免疫反应性（SP-LI）。2.通过交替电刺激切断的L7和S1前根的外周端，诱发小腿三头肌收缩5 - 9 min。基础SP-LI释放为0.20 +/-0.03 fmol/100微升，增加至0.54 +/-0.05 fmol/100微升（平均值+/-S.D.）通过静态肌肉收缩。在切断L7和S1背根后，这种增加大大减弱（0.23 +/-0.03至0.39 +/-0.08 fmol/100微升）或通过肌肉麻痹完全消除（0.27 +/-0.03至0.31 +/-0.01 fmol/100微升）。肌肉收缩还使平均动脉血压（MAP）增加29 +/-20 mmHg，心率（HR）增加11 +/-5次/分（平均值+/-S.D.）。通过切断背根或在猫麻痹后电刺激腹根，这些由肌肉收缩引起的心血管变化被消除。3.这些结果表明，骨骼肌的静态收缩增加了脊髓背角中SP-LI的释放。此外，这些数据支持的假设，SP起着介导的作用，在静态运动引起的心血管反应。
1. The purpose of this study was to determine if static skeletal muscle contraction causes the release of substance P(SP) in the L7‐dorsal horn region of the spinal cord. A laminectomy was performed to expose the spinal cord of alpha‐chloralose anaesthetized cats. The L6 spinal root was cut. A microdialysis probe was inserted into the L7 dorsal horn region ipsilateral to the contracting triceps surae muscle. The probe was perfused with a buffer solution at 3 microliters/min. Substance P‐like immunoreactivity (SP‐LI) was measured, from the microdialysis samples, by radioimmunoassay. 2. A 5‐9 min contraction of the triceps surae muscle was evoked by alternate electrical stimulation of the peripheral ends of the cut L7 and S1 ventral roots. Basal SP‐LI release was 0.20 +/‐ 0.03 fmol/100 microliters and was increased to 0.54 +/‐ 0.05 fmol/100 microliters (mean +/‐ S.D.) by static muscle contraction. This increase was greatly attenuated after cutting the L7 and S1 dorsal roots (0.23 +/‐ 0.03 to 0.39 +/‐ 0.08 fmol/100 microliters) or completely abolished by muscle paralysis (0.27 +/‐ 0.03 to 0.31 +/‐ 0.01 fmol/100 microliters). Muscle contraction also increased mean arterial blood pressure (MAP) 29 +/‐ 20 mmHg and heart rate (HR) 11 +/‐ 5 beats/min (mean +/‐ S.D.). These cardiovascular changes to muscle contraction were abolished by sectioning the dorsal roots or when the ventral roots were electrically stimulated after the cats were paralysed. 3. These results demonstrate that static contraction of skeletal muscle increases the release of SP‐LI in the dorsal horn of the spinal cord. Furthermore, these data support the hypothesis that SP plays a role in mediating the cardiovascular responses evoked during static exercise.
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影响因子：
4.8
作者：
HELKE, CJ;KRAUSE, JE;BANNON, MJ
通讯作者：
BANNON, MJ
骨骼肌的持续等长收缩导致麻醉猫脊髓中释放免疫反应性神经激肽
影响因子：
2.5
作者：
A. Duggan;P. J. Hope;C. W. Lang;C. Williams
通讯作者：
C. Williams
III 组肌肉传入对花生四烯酸静态收缩的敏感性。
DOI：
10.1152/jappl.1990.68.3.861
发表时间：
1990
期刊：
Journal of applied physiology (Bethesda, Md. : 1985)
影响因子：
--
作者：
Rotto,DM;Schultz,HD;Longhurst,JC;Kaufman,MP
通讯作者：
Kaufman,MP
DOI：
--
发表时间：
1971
期刊：
Journal of Physiology
影响因子：
--
作者：
By J. G. Coote;S. Hilton;J. Perez
通讯作者：
J. Perez
P 物质拮抗剂减弱对肌肉收缩的反射升压反应。
影响因子：
2.9
作者：
Kaufman,MP;Kozlowski,GP;Rybicki,KJ
通讯作者：
Rybicki,KJ