Comparative genomics and the role of lateral gene transfer in the evolution of bovine adapted Streptococcus agalactiae.
Comparative genomics and the role of lateral gene transfer in the evolution of bovine adapted Streptococcus agalactiae.
复制标题
比较基因组学和横向基因转移在牛适应无乳链球菌进化中的作用。
DOI：
10.1016/j.meegid.2011.04.019
复制
发表时间：
2011-08
影响因子：
3.2
通讯作者：
Stanhope, Michael J.
中科院分区：
文献类型：
--
作者：
Richards, Vincent P.;Lang, Ping;Bitar, Paulina D. Pavinski;Lefebure, Tristan;Schukken, Ynte H.;Zadoks, Ruth N.;Stanhope, Michael J.
除了在人类中引起严重的侵入性感染外，无乳链球菌或B组链球菌（GBS）也是牛乳腺炎的主要原因。在这里，我们提供了第一个基因组序列的S。从诊断为临床型乳腺炎的奶牛中分离的无乳链球菌（菌株FSL S3-026）。对比一下8个S。从人类疾病分离株中获得的无乳基因组显示了183个对牛菌株特异的基因。随后的聚合酶链反应（PCR）筛选在其他牛和人菌株中存在/不存在这些基因座的子集，显示两组之间存在很强的差异（Fisher精确检验：p < 0.0001）。大多数牛品系特异性基因（约85%）紧密聚集在8个基因组岛中，表明这些基因是通过横向基因转移（LGT）获得的。这牛GBS也包含了插入序列的比例异常高（总基因组的4.3%），这表明频繁的基因组重排。与其他乳腺炎致病菌种的比较为两例在共享牛环境中的种间LGT提供了强有力的证据：牛S。无乳链球菌与乳链球菌（乳链菌肽U操纵子）和停乳链球菌停乳（乳糖操纵子）。我们还发现了牛链球菌之间LGT的证据，涉及唾液素操纵子。无乳链球菌菌株和酿脓链球菌或唾液链球菌。我们的研究结果提供了对促进环境适应和获得潜在毒力因子的机制的深入了解，同时强调了LGT在牛S.无乳菌株，以及LGT在共享环境中的病原体中的重要性。
In addition to causing severe invasive infections in humans, Streptococcus agalactiae, or group B Streptococcus (GBS), is also a major cause of bovine mastitis. Here we provide the first genome sequence for S. agalactiae isolated from a cow diagnosed with clinical mastitis (strain FSL S3-026). Comparison to eight S. agalactiae genomes obtained from human disease isolates revealed 183 genes specific to the bovine strain. Subsequent polymerase chain reaction (PCR) screening for the presence/absence of a subset of these loci in additional bovine and human strains revealed strong differentiation between the two groups (Fisher exact test: p < 0.0001). The majority of the bovine strain-specific genes (~85%) clustered tightly into eight genomic islands, suggesting these genes were acquired through lateral gene transfer (LGT). This bovine GBS also contained an unusually high proportion of insertion sequences (4.3% of the total genome), suggesting frequent genomic rearrangement. Comparison to other mastitis-causing species of bacteria provided strong evidence for two cases of interspecies LGT within the shared bovine environment: bovine S. agalactiae with Streptococcus uberis (nisin U operon) and Streptococcus dysgalactiae subsp. dysgalactiae (lactose operon). We also found evidence for LGT, involving the salivaricin operon, between the bovine S. agalactiae strain and either Streptococcus pyogenes or Streptococcus salivarius. Our findings provide insight intomechanismsfacilitatingenvironmentaladaptationandacquisitionofpotential virulence factors, while highlighting both the key role LGT has played in the recent evolution of the bovine S. agalactiae strain, and the importance of LGT among pathogens within a shared environment.
登录
查看更多内容
影响因子：
4.9
作者：
Doherty, N;Trzcinski, K;Dowson, CG
通讯作者：
Dowson, CG
影响因子：
3.7
作者：
Beres SB;Musser JM
通讯作者：
Musser JM
影响因子：
3.2
作者：
Domelier, Anne-Sophie;van der Mee-Marquet, Nathalie;Quentin, Roland
通讯作者：
Quentin, Roland
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y
影响因子：
4.9
作者：
Brenciani, Andrea;Bacciaglia, Alessandro;Giovanetti, Eleonora
通讯作者：
Giovanetti, Eleonora