An overview of recent strategies in pathogen sensing.

An overview of recent strategies in pathogen sensing.
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DOI:
10.3390/s90604483
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发表时间:
2009
期刊:
Sensors (Basel, Switzerland)
影响因子:
--
通讯作者:
Hua SZ
Hua SZ
中科院分区:
其他
文献类型:
--
作者:
Heo J;Hua SZ

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致病菌是食品工业和水处理设施中主要关注的问题之一,因为它们的快速生长和对人类健康的有害影响。开发快速、准确的菌株检测和鉴定系统一直是研究人员关注的重要问题。虽然对细菌的鉴定是肯定的,但传统方法需要耗时的过程,包括对特征代谢物或细胞生殖周期的测试。本文综述了近年来基于微纳米加工技术的传感策略。这些技术大大提高了检测限,从而减少了样品制备所需的时间。本文将重点介绍新开发的纳米和微尺度生物传感器,利用微流控芯片实验室的新型传感方式,以及用于检测致病菌的阵列技术。
Pathogenic bacteria are one of the major concerns in food industries and water treatment facilities because of their rapid growth and deleterious effects on human health. The development of fast and accurate detection and identification systems for bacterial strains has long been an important issue to researchers. Although confirmative for the identification of bacteria, conventional methods require time-consuming process involving either the test of characteristic metabolites or cellular reproductive cycles. In this paper, we review recent sensing strategies based on micro- and nano-fabrication technology. These technologies allow for a great improvement of detection limit, therefore, reduce the time required for sample preparation. The paper will be focused on newly developed nano- and micro-scaled biosensors, novel sensing modalities utilizing microfluidic lab-on-a-chip, and array technology for the detection of pathogenic bacteria.
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