Solvent optimization on Taxol extraction from Taxus baccata L., using HPLC and LC-MS
Solvent optimization on Taxol extraction from Taxus baccata L., using HPLC and LC-MS
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使用 HPLC 和 LC-MS 优化红豆杉中紫杉醇提取的溶剂
DOI：
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发表时间：
2009
期刊：
影响因子：
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通讯作者：
A. Esmaeili
中科院分区：
文献类型：
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作者：
H. Sadeghi;G. Asghari;S. A. Mostafavi;A. Esmaeili
背景与研究目的：紫杉醇是从短叶红豆杉树皮提取物中首次分离得到的天然抗肿瘤药物。，这可能是紫杉醇的有限来源。为了寻找红豆杉针叶中紫杉醇的替代来源、最佳提取溶剂和成本效益较高的提取溶剂，采用不同比例的溶剂从红豆杉针叶中提取紫杉醇。方法：采集来自伊朗托尔卡曼和努尔市的巴卡红豆杉干燥针叶1g，分别用无水乙醇或丙酮和50%和20%的乙醇或丙酮水提取。将溶剂蒸发至干燥，残留物溶解在5毫升甲醇中并过滤。每毫升滤液中加入50μ的乙酸桂皮酯L溶液作为内标，20μ的L溶液经高效液相色谱测定被试溶剂的萃取效率。以水/乙腈(10/90)为流动相，正电喷雾电离鉴定紫杉醇的真伪，同时对5个μL的滤液进行LC-MS分析。结果：紫杉醇的提取效率随乙醇或丙酮比例的增加而提高。高效液相色谱分析表明，紫杉醇可以用标准曲线进行紫外检测。标准曲线的线性范围为7.80~500μg/ml(R2=0.9992)，变异系数为0.52~15.36。阳离子模式下的LC-MS分析证实了紫杉醇(m/z 854；M+H)的真实性，以及M+Na(m/z 876)、M+K(m/z 892)和M+CH3CN+H2O(m/z 913)等加合离子的存在。结论：红豆杉针叶中紫杉醇的最佳提取溶剂为100%丙酮。
Background and the purpose of the study: Taxol, a natural antitumor agent, was first isolated from the extract of the bark of Taxus brevifolia Nutt . , which is potentially a limited source for Taxol. In the search of an alternative source, optimum and cost benefit extracting solvents, various solvents with different percentage were utilized to extract Taxol from needles of Taxus baccata . Methods: One g of the dried needles of Taxus baccata , collected from Torkaman and Noor cities of Iran, was extracted with pure ethanol or acetone and 50% and 20% of ethanol or acetone in water. Solvents were evaporated to dryness and the residues were dissolved in 5 ml of methanol and filtered. To one ml of the filtrate was added 50 μl of cinamyl acetate as the internal standard and 20 μl of the resulting solution was subjected to the HPLC to determine the extraction efficiencies of tested solvents. Five μl of filtrate was also subjected to the LC-MS using water/acetonitrile (10/90) as mobile phase and applying positive electrospray ionization (ESI) to identify the authenticity of Taxol. Results: Results of this study indicated that Taxol extraction efficiency was enhanced as the percentage of ethanol or acetone was increased. HPLC analysis showed that Taxol could be quantified by UV detection using standard curve. The standard curve covering the concentration ranges of 7.8 - 500 μg/ml was linear (r2= 0.9992) and CV% ranged from 0.52 to 15.36. LC-MS analysis using ESI in positive-ion mode confirmed the authenticity of Taxol (m/z 854; M+H), as well as some adduct ions such as M+Na (m/z 876), M+K (m/z 892) and M+CH3CN+H2O (m/z 913). Conclusions: The results suggest that 100% acetone is the best solvent for the extraction of Taxol from Taxus baccata needles.
影响因子：
5.1
作者：
WHEELER, NC;JECH, K;SNADER, KM
通讯作者：
SNADER, KM