Electrical Access to Lipid Bilayer Membrane Microchambers for Transmembrane Analysis
Electrical Access to Lipid Bilayer Membrane Microchambers for Transmembrane Analysis
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用于跨膜分析的脂质双层膜微室的电气接入
DOI：
10.1109/jmems.2011.2153826
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发表时间：
2011
影响因子：
2.7
通讯作者：
S. Takeuchi
中科院分区：
文献类型：
--
作者：
T. Osaki;Y. Watanabe;R. Kawano;H. Sasaki;S. Takeuchi
这封信描述了集成的电连接到脂质双层膜微芯片的电检测/调节跨膜现象连同荧光监测。在聚（二甲基硅氧烷）微流体装置内制造具有3 pL体积的阵列微室，并且通过设计与由玻璃基板上的金图案桥接的室相邻的通道来实现电接入。我们成功地在腔室的口处形成脂质双层膜，并向具有集成组件的膜施加电势。我们通过用电连接的信号监测证实了跨膜纳米孔蛋白到形成的脂质双层膜中的重构。
This letter describes the integration of an electrical connection onto a lipid bilayer membrane microchip for the electrical detection/regulation of transmembrane phenomena together with fluorescence monitoring. Arrayed microchambers with a volume of 3 pL were fabricated within a poly(dimethyl siloxane) microfluidic device, and electrical access was achieved by designing channels adjacent to the chambers that were bridged by gold patterns on a glass substrate. We succeeded in the formation of a lipid bilayer membrane at the mouth of the chamber and applied electrical potential to the membrane with the integrated component. We confirmed the reconstitution of a transmembrane nanopore protein into the formed lipid bilayer membrane by signal monitoring with the electrical connection.
影响因子：
15
作者：
Heron, Andrew J.;Thompson, James R.;Wallace, Mark I.
通讯作者：
Wallace, Mark I.