Gaussian vs. Bessel light-sheets: performance analysis in live large sample imaging
Gaussian vs. Bessel light-sheets: performance analysis in live large sample imaging
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高斯光片与贝塞尔光片：实时大样本成像中的性能分析
DOI：
10.1117/12.2277324
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Donnachie M
中科院分区：
文献类型：
--
作者：
Donnachie M
光片荧光显微镜（LSFM）在过去的十年中迅速发展，从一个新兴的技术成为一个既定的方法。这一进展在很大程度上是由其对发育生物学的适用性所驱动的，在发育生物学中，它能够在相对较大的视场内提供优异的时空分辨率，具有良好的对比度和低的光毒性。在许多方面，它正在取代共聚焦显微镜。然而，它不是灵丹妙药，仍然难以在更高散射的样品中深入成像。已经提出了许多解决方案，包括Airy和Bessel照明，双光子操作和去卷积技术。在这项工作中，我们展示了一个简单但有效的高斯光束照明和贝塞尔照明鸡胚成像之间的比较。虽然贝塞尔照明被证明是有益的，当一个更大的景深是必需的，它是不可能看到任何好处的鸡胚的高度散射组织成像。
Lightsheet fluorescence microscopy (LSFM) has rapidly progressed in the past decade from an emerging technology into an established methodology. This progress has largely been driven by its suitability to developmental biology, where it is able to give excellent spatial-temporal resolution over relatively large fields of view with good contrast and low phototoxicity. In many respects it is superseding confocal microscopy. However, it is no magic bullet and still struggles to image deeply in more highly scattering samples. Many solutions to this challenge have been presented, including, Airy and Bessel illumination, 2-photon operation and deconvolution techniques. In this work, we show a comparison between a simple but effective Gaussian beam illumination and Bessel illumination for imaging in chicken embryos. Whilst Bessel illumination is shown to be of benefit when a greater depth of field is required, it is not possible to see any benefits for imaging into the highly scattering tissue of the chick embryo.
偏振光片断层扫描。
影响因子：
3.8
作者：
Sascha Reidt;Daniel J. O'Brien;K. Wood;M. MacDonald
通讯作者：
M. MacDonald