Upregulation of lncRNA147410.3 in the Brain of Mice With Chronic Toxoplasma Infection Promoted Microglia Apoptosis by Regulating Hoxb3.
Upregulation of lncRNA147410.3 in the Brain of Mice With Chronic Toxoplasma Infection Promoted Microglia Apoptosis by Regulating Hoxb3.
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DOI：
10.3389/fncel.2021.648047
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发表时间：
2021
影响因子：
5.3
通讯作者：
Cong H
中科院分区：
文献类型：
--
作者：
Wang Y;Han R;Xu Z;Sun X;Zhou C;Han B;He S;Cong H
弓形虫具有嗜神经性，影响神经细胞的功能，但其作用机制尚不清楚。LncRNAs在大脑中含量丰富，参与了中枢神经系统(CNS)发育的精细调节。然而，这些lncRNAs是否参与了弓形虫感染过程中小胶质细胞的激活调控，目前尚不清楚。在本研究中，一个新的LncRNA147410.3(ENSMUST00000147410.3)的上调被认为是影响这一过程的关键因素。经预测，LncRNA147410.3的目的基因为Hoxb3。FISH实验证实lncRNA147410.3定位于脑和神经胶质细胞的胞核。进一步证实了lncRNA147410.3对神经细胞的作用，它可能通过正向调节Hoxb3而影响小鼠小胶质细胞的增殖、分化和凋亡。因此，本研究探讨了lncRNA147410.3在弓形虫感染小鼠脑内的调节作用，为利用lncRNA147410.3作为治疗靶点治疗弓形虫所致神经功能障碍提供科学依据。
Toxoplasma gondii is neurotropic and affects the function of nerve cells, while the mechanism is unclear. LncRNAs are abundantly enriched in the brain and participated in the delicate regulation of the central nervous system (CNS) development. However, whether these lncRNAs are involved in the regulation of microglia activation during the process of T. gondii infection is largely unknown. In this study, the upregulation of a novel lncRNA147410.3 (ENSMUST00000147410.3) was identified as a key factor to influence this process. The target gene of lncRNA147410.3 was predicted and identified as Hoxb3. The localization of lncRNA147410.3 in the brain and cells was proved in the nucleus of neuroglia through FISH assay. Furthermore, the function of lncRNA147410.3 on neuronal cell was confirmed that lncRNA147410.3 could affect proliferation, differentiation, and apoptosis of mouse microglia by positively regulating Hoxb3. Thus, our study explored the modulatory action of lncRNA147410.3 in T. gondii infected mouse brain, providing a scientific basis for using lncRNA147410.3 as a therapeutic target to treat neurological disorder induced by T. gondii.
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