Pexophagy suppresses ROS-induced damage in leaf cells under high-intensity light.
Pexophagy suppresses ROS-induced damage in leaf cells under high-intensity light.
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DOI：
10.1038/s41467-022-35138-z
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发表时间：
2022-12-05
影响因子：
16.6
通讯作者：
Nishimura, Mikio
中科院分区：
文献类型：
--
作者：
Oikawa, Kazusato;Goto-Yamada, Shino;Hayashi, Yasuko;Takahashi, Daisuke;Kimori, Yoshitaka;Shibata, Michitaro;Yoshimoto, Kohki;Takemiya, Atsushi;Kondo, Maki;Hikino, Kazumi;Kato, Akira;Shimoda, Keisuke;Ueda, Haruko;Uemura, Matsuo;Numata, Keiji;Ohsumi, Yoshinori;Hara-Nishimura, Ikuko;Mano, Shoji;Yamada, Kenji;Nishimura, Mikio
虽然光是光合作用所必需的，但它有可能提高细胞内活性氧（ROS）的水平。由于高水平的ROS具有细胞毒性，植物必须减轻这种损害。然而，ROS诱导的过氧化物酶体叶片损伤缓解的细胞机制尚未完全探讨。在这里，我们表明，自噬在选择性去除ROS产生的过氧化物酶体，保护植物免受光合作用过程中的氧化损伤中起着至关重要的作用。我们提出的证据表明，自噬缺陷突变体表现出光强度依赖性的叶片损伤和过量聚集的ROS积累过氧化物酶体。过氧化物酶体聚集体特异性地被叶细胞和孤立的液泡中的前自噬体结构和液泡膜吞噬，但它们在突变体中不降解。ATG 18 a-GFP和GFP-2×FYVE与磷脂酰肌醇3-磷酸结合，在高强度光下优先靶向ROS积累细胞中过氧化物酶体膜和过氧化物酶体附近的前自噬体结构。我们的研究结果提供了更深入的见解，植物胁迫反应引起的光照射。Pexophagy在选择性去除ROS产生的过氧化物酶体中起着关键作用，该过氧化物酶体在高强度光下的光合作用期间保护植物免受氧化损伤。
Although light is essential for photosynthesis, it has the potential to elevate intracellular levels of reactive oxygen species (ROS). Since high ROS levels are cytotoxic, plants must alleviate such damage. However, the cellular mechanism underlying ROS-induced leaf damage alleviation in peroxisomes was not fully explored. Here, we show that autophagy plays a pivotal role in the selective removal of ROS-generating peroxisomes, which protects plants from oxidative damage during photosynthesis. We present evidence that autophagy-deficient mutants show light intensity-dependent leaf damage and excess aggregation of ROS-accumulating peroxisomes. The peroxisome aggregates are specifically engulfed by pre-autophagosomal structures and vacuolar membranes in both leaf cells and isolated vacuoles, but they are not degraded in mutants. ATG18a-GFP and GFP-2×FYVE, which bind to phosphatidylinositol 3-phosphate, preferentially target the peroxisomal membranes and pre-autophagosomal structures near peroxisomes in ROS-accumulating cells under high-intensity light. Our findings provide deeper insights into the plant stress response caused by light irradiation. Pexophagy plays a pivotal role in the selective removal of ROS-generating peroxisomes, which protects plants from oxidative damage during photosynthesis under high-intensity light.
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影响因子：
3.5
作者：
Krick, Roswitha;Tolstrup, Joern;Thumm, Michael
通讯作者：
Thumm, Michael
DOI：
10.1073/pnas.1511570112
发表时间：
2015-08-11
影响因子：
11.1
作者：
Brunkard, Jacob O.;Runkel, Anne M.;Zambryski, Patricia C.
通讯作者：
Zambryski, Patricia C.
DOI：
10.1083/jcb.200412022
发表时间：
2005-05-09
期刊：
The Journal of cell biology
影响因子：
--
作者：
Komatsu M;Waguri S;Ueno T;Iwata J;Murata S;Tanida I;Ezaki J;Mizushima N;Ohsumi Y;Uchiyama Y;Kominami E;Tanaka K;Chiba T
通讯作者：
Chiba T
DOI：
10.1038/nrm.2016.74
发表时间：
2016-09
期刊：
Nature reviews. Molecular cell biology
影响因子：
--
作者：
Farré JC;Subramani S
通讯作者：
Subramani S
影响因子：
3.9
作者：
FEIERABEND, J;ENGEL, S
通讯作者：
ENGEL, S