Comprehensive analysis of DOK family genes expression, immune characteristics, and drug sensitivity in human tumors.
Comprehensive analysis of DOK family genes expression, immune characteristics, and drug sensitivity in human tumors.
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人类肿瘤中DOK家族基因表达、免疫特征及药物敏感性综合分析
DOI：
10.1016/j.jare.2021.06.008
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发表时间：
2022-03
影响因子：
10.7
通讯作者：
Wang W
中科院分区：
文献类型：
--
作者：
Guan Y;Li M;Qiu Z;Xu J;Zhang Y;Hu N;Zhang X;Guo W;Yuan J;Shi Q;Wang W
DOK家族基因的表达与总生存期（OS），临床阶段，肿瘤突变，甲基化，CNV和SNV有关。
DOK家族基因与UVM预后不良显着相关。
DOK1-DOK3与肿瘤免疫和肿瘤微环境具有明显的相关性。
DOK家族基因与肿瘤干和药物敏感性显着相关。
DOK家族基因的表达与EMT和激素ER途径的激活有关，并且与DNA损伤反应，细胞周期和激素AR途径的抑制有关。
DOK1和DOK3，DOK2和DOK3具有显着的相关性。
DOK是一种新型的调节蛋白家族，参与调节肿瘤细胞生长。但是，大多数研究都是在细胞系中进行的，并且在人类肿瘤中尚未进行系统研究。
我们根据DOK的表达谱以及与患者生存，免疫浸润，肿瘤微环境和药物敏感性的关系进行了全面的分析。
我们使用TCGA数据库分析了DOK家族基因表达与预后和临床阶段之间的相关性。通过免疫组织化学分析了DOK在肿瘤组织中的蛋白质表达。使用CBIOPORTAL数据库分析人类肿瘤中DOK家族基因的变化频率。此外，我们使用估计算法和计时器网站来分析DOK家族基因与肿瘤免疫力之间的相关性。最后，我们进一步分析了DOK家族基因与肿瘤干与癌细胞对化学疗法的敏感性之间的关系。
我们根据DOK家族基因的表达谱及其与患者生存的关系进行了全面的分析。我们还通过免疫组织化学证实了这一结论。 DOK家族基因的表达与OS，临床阶段，肿瘤突变，甲基化，CNV和SNV有关。 DOK家族基因与UVM预后不良显着相关。 DOK1-DOK3与肿瘤免疫有明显的相关性。 DOK2可以增加化学疗法药物的敏感性，而DOK4降低了多种化疗药物的敏感性。另外，DOK家族基因的表达水平与癌症标记相关途径的活性显着相关。
DOK在不同肿瘤中扮演肿瘤抑制基因或促肿瘤基因的作用。但是，DOK家族基因在促进UVM中的癌症方面发挥了作用。 DOK家族基因与药物敏感性显着相关。
The expression of DOK family genes is related to overall survival (OS), clinical stage, tumor mutation, methylation, CNV, and SNV. DOK family genes are significantly associated with poor prognosis of UVM. DOK1-DOK3 has obvious correlation with tumor immunity and tumor microenvironment. DOK family gene is significantly related to tumor stemness and drug sensitivity. The expression of DOK family genes is related to the activation of EMT and hormone ER pathways, and is related to the inhibition of DNA damage response, cell cycle, and hormone AR pathways. DOK1 and DOK3, DOK2 and DOK3 have the significant correlation. DOK is a new type of regulatory protein family that participates in the regulation of tumor cell growth. However, most of the studies are conducted in cell lines, and systematic studies have not been conducted in human tumors. We conducted a comprehensive analysis of DOK based on its expression profile and its relationship with patient survival, immune infiltration, tumor microenvironment, and drug sensitivity. We used the TCGA database to analyze the correlation between DOK family gene expression and prognosis and clinical stage. The protein expression of DOK in tumor tissues was analyzed by immunohistochemistry. Use the cBioPortal database to analyze the alteration frequency in DOK family genes in human tumors. In addition, we used ESTIMATE algorithm and TIMER website to analyze the correlation between DOK family genes and tumor immunity. Finally, we further analyzed the relationship between DOK family genes and tumor stemness and the sensitivity of cancer cells to chemotherapy. We conducted a comprehensive analysis of DOK family genes based on its expression profile and its relationship with patient survival. We also confirmed this conclusion by immunohistochemistry. The expression of DOK family genes is related to OS, clinical stage, tumor mutation, methylation, CNV, and SNV. DOK family genes are significantly associated with poor prognosis of UVM. DOK1-DOK3 has obvious correlation with tumor immunity. DOK2 can increase the sensitivity of chemotherapy drugs, while DOK4 reduces the sensitivity of multiple chemotherapy drugs. In addition, the expression level of DOK family genes is significantly correlated with the activity of cancer marker-related pathways. DOK plays a role of tumor suppressor gene or tumor-promoting gene in different tumors. However, DOK family genes play a role in promoting cancer in UVM. DOK family genes are significantly associated with drug sensitivity.
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影响因子：
2.2
作者：
Bevilacqua JA;Lara M;Díaz J;Campero M;Vázquez J;Maselli RA
通讯作者：
Maselli RA
乳腺癌不一致同卵双胞胎的 DNA 甲基化分析将 DOK7 确定为新型表观遗传生物标志物。
影响因子：
4.7
作者：
Heyn H;Carmona FJ;Gomez A;Ferreira HJ;Bell JT;Sayols S;Ward K;Stefansson OA;Moran S;Sandoval J;Eyfjord JE;Spector TD;Esteller M
通讯作者：
Esteller M
影响因子：
11.1
作者：
Friedrich T;Söhn M;Gutting T;Janssen KP;Behrens HM;Röcken C;Ebert MPA;Burgermeister E
通讯作者：
Burgermeister E
影响因子：
4.4
作者：
Gerard, Audrey;Ghiotto, Marguerite;Nunes, Jacques A.
通讯作者：
Nunes, Jacques A.
影响因子：
19
作者：
Klemm F;Joyce JA
通讯作者：
Joyce JA