Multiplex One-Step RT-qPCR Assays for Simultaneous Detection of SARS-CoV-2 and Other Enteric Viruses of Dogs and Cats.
Multiplex One-Step RT-qPCR Assays for Simultaneous Detection of SARS-CoV-2 and Other Enteric Viruses of Dogs and Cats.
复制标题
DOI：
10.3390/v15091890
复制
发表时间：
2023-09-07
期刊：
影响因子：
--
通讯作者：
Balasuriya UBR
中科院分区：
文献类型：
--
作者：
Thieulent CJ;Carossino M;Peak L;Wolfson W;Balasuriya UBR
在COVID-19大流行期间，严重急性呼吸综合征冠状病毒-2 （SARS-CoV-2）从人传播给狗和猫（反向人畜共患病）。在受感染的狗和猫的粪便样本中检测到SARS-CoV-2，表明可能存在粪口传播、环境污染和人畜共患传播（即溢出）。此外，胃肠道病毒感染在狗和猫中很普遍。在本研究中，我们建立并验证了一套同时检测SARS-CoV-2和常见犬肠道病毒的多重一步逆转录-定量聚合酶链反应（RT-qPCR）试剂盒：用于检测犬腺病毒-1、犬肠道冠状病毒、犬瘟热病毒和犬细小病毒的犬肠道Assay_1 （CEA_1）和用于检测轮状病毒a （RVA）和SARS-CoV-2的CEA_2；或常见的猫肠道病毒（用于检测猫肠道冠状病毒、猫泛白细胞减少病毒、RVA和SARS-CoV-2的猫肠道检测试剂盒）。所有的检测都显示出很高的分析灵敏度，在多重格式下检测到5-35个基因组拷贝/µL。该方法重复性好，重现性好，变异系数<4%。在检测的58份临床样本中，34.5%的样本至少对其中一种病毒呈阳性，分别从一只狗和一只猫身上采集的两份样本中检测到SARS-CoV-2。总之，这些新开发的一步多重RT-qPCR检测方法可以快速诊断狗和猫的肠道病毒感染，包括SARS-CoV-2。
The severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) was transmitted from humans to dogs and cats (reverse zoonosis) during the COVID-19 pandemic. SARS-CoV-2 has been detected in fecal samples of infected dogs and cats, indicating potential fecal–oral transmission, environmental contamination, and zoonotic transmission (i.e., spillback). Additionally, gastrointestinal viral infections are prevalent in dogs and cats. In this study, we developed and validated a panel of multiplex one-step reverse transcription–quantitative polymerase chain reaction (RT-qPCR) assays for the simultaneous detection of SARS-CoV-2 and common canine enteric viruses: Canine Enteric Assay_1 (CEA_1) for the detection of canine adenovirus-1, canine enteric coronavirus, canine distemper virus, and canine parvovirus, and CEA_2 for the detection of rotavirus A (RVA), and SARS-CoV-2); or common feline enteric viruses (Feline Enteric Assay_1 (FEA_1) for the detection of feline enteric coronavirus, feline panleukopenia virus, RVA, and SARS-CoV-2). All assays demonstrated high analytical sensitivity, detecting as few as 5–35 genome copies/µL in multiplex format. The repeatability and reproducibility of the multiplex assays were excellent, with coefficient of variation <4%. Among the 58 clinical samples tested, 34.5% were positive for at least one of these viruses, and SARS-CoV-2 was detected in two samples collected from one dog and one cat, respectively. In conclusion, these newly developed one-step multiplex RT-qPCR assays allow for rapid diagnosis of enteric viral infections, including SARS-CoV-2, in dogs and cats.
登录
查看更多内容
影响因子：
4.6
作者：
通讯作者：
--
影响因子：
4.8
作者：
Carossino, Mariano;Barrandeguy, Maria E.;Balasuriya, Udeni B. R.
通讯作者：
Balasuriya, Udeni B. R.
影响因子：
3.1
作者：
Dowgier, Giulia;Mari, Viviana;Decaro, Nicola
通讯作者：
Decaro, Nicola
影响因子：
3.1
作者：
Decaro N;Pratelli A;Campolo M;Elia G;Martella V;Tempesta M;Buonavoglia C
通讯作者：
Buonavoglia C
影响因子：
3.3
作者：
Duijvestijn M;Mughini-Gras L;Schuurman N;Schijf W;Wagenaar JA;Egberink H
通讯作者：
Egberink H