Enteropathogen infections in canine puppies: (Co-)occurrence, clinical relevance and risk factors.
Enteropathogen infections in canine puppies: (Co-)occurrence, clinical relevance and risk factors.
复制标题
DOI：
10.1016/j.vetmic.2016.09.006
复制
发表时间：
2016-11-15
影响因子：
3.3
通讯作者：
Egberink H
中科院分区：
文献类型：
--
作者：
Duijvestijn M;Mughini-Gras L;Schuurman N;Schijf W;Wagenaar JA;Egberink H
CPV、CCoV和β-溶血性E.大肠杆菌与幼犬的（严重）急性腹泻有关。来自高容量繁殖者的幼犬更有可能感染CPV、CCoV和囊等孢菌属。CPV和CCoV在冬季/春季更常见。多重肠道感染在有或没有腹泻的幼犬中很常见。CPV、CCoV和β-溶血性E.大肠杆菌，建议在腹泻的小狗。幼犬腹泻病原体的实验室确认可能允许适当的治疗。然而，潜在病原体的存在并不能证明与腹泻存在因果关系。本研究的目的是在≤12月龄幼犬中识别有和无急性腹泻的特定肠道病原体，并评估其与临床体征、推定风险因素和病原体共存的相关性。收集来自患有（n = 113）和未患有（n = 56）急性腹泻的幼犬的粪便样品，并针对犬细小病毒（CPV）、犬冠状病毒（CCoV）、沙门氏菌属、弯曲杆菌属，产气荚膜梭菌、艰难梭菌、β-溶血性土杆菌（hEC）、贾第虫属，弓蛔虫属，囊等孢菌属，和Cyniclomyces guttulatus。在86.5%的腹泻幼犬和77.8%的无症状幼犬中检测到一种或多种病原体。CPV与CCoV、CPV与Cystoisospora spp.、弓蛔虫属和hEC、贾第虫属（Giardia spp.）和C.点滴状只有CPV和CCoV与腹泻显著相关，hEC与腹泻和严重临床体征的幼犬子集相关。CPV在3月龄以下的幼犬中更为流行。来自高容量犬育种者的幼犬患CPV（OR 4.20）、CCoV（OR 4.50）和囊等孢菌属的风险显著增加。(OR 3.60）。与夏季相比，CCoV在冬季（OR 3.35）发生率显著更高，CPV在冬季（OR 3.78）和春季（OR 4.72）发生率显著更高。我们的结论是，建议对患有急性腹泻的幼犬进行CPV，CCoV和hEC的常规筛查，特别是如果它们年龄在3个月以下并且来自高容量犬育种者。其他病原体的常规筛查可能导致不太确定的结果。
CPV, CCoV and β-hemolytic E. coli are associated with (severe) acute diarrhoea in puppies. Puppies from high-volume breeders are more likely to be infected with CPV, CCoV and Cystoisospora spp. CPV and CCoV are more often detected in winter/spring. Multiple enteric infections are common in puppies with and without diarrhoea. Screening of CPV, CCoV and β-hemolytic E. coli is advised in diarrhoeic puppies. Laboratory confirmation of the causative agent(s) of diarrhoea in puppies may allow for appropriate treatment. The presence of potential pathogens however, does not prove a causal relationship with diarrhoea. The aim of this study was to identify specific enteropathogens in ≤12 month old puppies with and without acute diarrhoea and to assess their associations with clinical signs, putative risk factors and pathogen co-occurrence. Faecal samples from puppies with (n = 113) and without (n = 56) acute diarrhoea were collected and screened for Canine Parvovirus (CPV), Canine Coronavirus (CCoV), Salmonella spp., Campylobacter spp., Clostridium perfringens, Clostridium difficile, β-hemolytic Eschericha coli (hEC), Giardia spp., Toxocara spp., Cystoisospora spp., and Cyniclomyces guttulatus. One or more pathogens were detected in 86.5% of diarrhoeic puppies and in 77.8% of asymptomatic puppies. Significant positive associations were found between CPV and CCoV, CPV and Cystoisospora spp., Toxocara spp. and hEC, Giardia spp. and C. guttulatus. Only CPV and CCoV were significantly associated with diarrhoea, hEC with a subset of puppies that had diarrhoea and severe clinical signs. CPV was more prevalent in puppies under 3 months of age. Puppies from high-volume dog breeders were significantly at increased risk for CPV (OR 4.20), CCoV (OR 4.50) and Cystoisospora spp. (OR 3.60). CCoV occurred significantly more often in winter (OR 3.35), and CPV in winter (OR 3.78) and spring (OR 4.72) as compared to summer. We conclude that routine screening for CPV, CCoV and hEC is recommended in puppies with acute diarrhoea, especially if they are under 3 months of age and originate from high-volume dog breeders. Routine screening for other pathogens may lead to less conclusive results.
登录
查看更多内容
影响因子：
2.2
作者：
Decaro, Nicola;Desario, Costantina;Buonavoglia, Canio
通讯作者：
Buonavoglia, Canio
影响因子：
3.1
作者：
Decaro N;Pratelli A;Campolo M;Elia G;Martella V;Tempesta M;Buonavoglia C
通讯作者：
Buonavoglia C
影响因子：
9.4
作者：
Helps, C;Reeves, N;Harbour, D
通讯作者：
Harbour, D
影响因子：
3.3
作者：
Decaro N;Buonavoglia C
通讯作者：
Buonavoglia C
影响因子：
2.2
作者：
Decaro, Nicola;Desario, Costantina;Buonavoglia, Canio
通讯作者：
Buonavoglia, Canio