Evaluation of Polyacrylonitrile Nonwoven Mats and Silver-Gold Bimetallic Nanoparticle-Decorated Nonwoven Mats for Potential Promotion of Wound Healing In Vitro and In Vivo and Bone Growth In Vitro.
Evaluation of Polyacrylonitrile Nonwoven Mats and Silver-Gold Bimetallic Nanoparticle-Decorated Nonwoven Mats for Potential Promotion of Wound Healing In Vitro and In Vivo and Bone Growth In Vitro.
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DOI：
10.3390/polym13040516
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发表时间：
2021-02-09
期刊：
影响因子：
5
通讯作者：
Wu CC
中科院分区：
文献类型：
--
作者：
Bai MY;Ku FY;Shyu JF;Hayashi T;Wu CC
我们采用静电纺丝技术制备了聚丙烯腈（PAN）和海胆状银-金双金属或银纳米粒子装饰的PAN无纺垫，并在体外和体内评估了它们对伤口愈合、皮肤组织抗菌作用以及体外促进骨骼长入的作用。为了获得这些非织造垫，开发了一种简便、绿色、低温的方案。处理5小时后，类海胆银-金双金属和银纳米粒子修饰的PAN非织造垫对金黄色葡萄球菌的杀菌率分别为96.81±2.81%和51.90±9.07%。在体外细胞模型中，这两种垫没有表现出明显的毒性；处理5小时内细胞活力>80%。体内动物模型临床前评估表明，由于皮肤组织再生过程中皮脂腺、毛囊和脂肪的形成，海胆样银金双金属非织造垫组显示出显着的伤口恢复；在真皮层中观察到新血管形成增加和胶原纤维致密，与对照组的结果相当。海胆状银金双金属纳米粒子修饰的PAN无纺垫的母体，即纯PAN无纺垫，被发现是骨组织工程的潜在支架，因为观察到成骨细胞从膜的顶部到底部向内生长以及膜内的增殖。关键遗传因子Cbfa1在体外被鉴定为关键的成骨细胞分化调节因子。因此，电纺膜材料显示出用作骨再生和感染控制的双功能生物材料以及用于感染性骨和软组织缺陷的复合移植物的潜力。
We prepared polyacrylonitrile (PAN) and urchin-like Ag–Au bimetallic or Ag nanoparticle-decorated PAN nonwoven mats using electrospinning and evaluated them in vitro and in vivo for wound healing, antibacterial effects on skin tissue, and promotion of bone ingrowth in vitro. A facile, green, low-temperature protocol was developed to obtain these nonwoven mats. The sterilization rate of urchin-like Ag–Au bimetallic and Ag nanoparticle-decorated PAN nonwoven mats against Staphylococcus aureus was 96.81 ± 2.81% and 51.90 ± 9.07%, respectively, after 5 h treatment. In an in vitro cell model, these two mats did not show significant toxicity; cell viability of >80% was obtained within 5 h of treatment. In vivo animal model preclinical assessment showed that the urchin-like Ag–Au bimetallic nonwoven mat group showed significant wound recovery because of sebaceous gland, hair follicle, and fat formation during skin tissue regeneration; increased neovascularization and compact collagen fibers were observed in the dermal layer, comparable to the findings for the control group. The mother substrate of the urchin-like Ag–Au bimetallic nanoparticle-decorated PAN nonwoven mats, that is, pure PAN nonwoven mats, was found to be a potential scaffold for bone tissue engineering as osteoblast ingrowth from the top to the bottom of the membrane and proliferation inside the membrane were observed. The key genetic factor Cbfa1 was identified as a key osteoblast differentiation regulator in vitro. Thus, electrospun membrane materials show potential for use as dual-functional biomaterials for bone regeneration and infection control and composite grafts for infectious bone and soft tissue defects.
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影响因子：
5.6
作者：
Gao C;Deng Y;Feng P;Mao Z;Li P;Yang B;Deng J;Cao Y;Shuai C;Peng S
通讯作者：
Peng S
影响因子：
3.9
作者：
Trabelsi, Marah;Al Mamun;Ehrmann, Andrea
通讯作者：
Ehrmann, Andrea
影响因子：
8.6
作者：
Yun, Ki Myoung;Suryamas, Adi Bagus;Okuyama, Kikuo
通讯作者：
Okuyama, Kikuo
影响因子：
1.2
作者：
Maneewattanapinyo, Pattwat;Banlunara, Wijit;Kaewamatawong, Theerayuth
通讯作者：
Kaewamatawong, Theerayuth
影响因子：
56.9
作者：
LANGER, R;VACANTI, JP
通讯作者：
VACANTI, JP