A genetically encoded photocaged Nepsilon-methyl-L-lysine.
A genetically encoded photocaged Nepsilon-methyl-L-lysine.
复制标题
基因编码的光笼 Nepsilon-甲基-L-赖氨酸。
作者:
Yane;Bo Wu;Zhiyong Wang;Ying Huang;W. Wan;W. Russell;Pei;Yin N Moe;D. Russell;W. Liu
A photocaged N(epsilon)-methyl-L-lysine has been genetically incorporated into proteins at amber codon positions in Escherichia coli using an evolved pyrrolysyl-tRNA synthetase-pylT pair. Its genetic incorporation and following photolysis to recover N(epsilon)-methyl-L-lysine at physiological pH provide a convenient method for the biosynthesis of proteins with monomethylated lysines at specific sites.
影响因子:
16
作者:
Yang, Xiang-Jiao;Seto, Edward
通讯作者:
Seto, Edward
影响因子:
16.6
作者:
Fekner, Tomasz;Li, Xin;Chan, Michael K.
通讯作者:
Chan, Michael K.