Only Acyl Carrier Protein 1 (AcpP1) Functions in Pseudomonas aeruginosa Fatty Acid Synthesis.
Only Acyl Carrier Protein 1 (AcpP1) Functions in Pseudomonas aeruginosa Fatty Acid Synthesis.
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只有酰基载体蛋白 1 (AcpP1) 在铜绿假单胞菌脂肪酸合成中起作用
DOI：
10.3389/fmicb.2017.02186
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发表时间：
2017
影响因子：
5.2
通讯作者：
Wang HH
中科院分区：
文献类型：
--
作者：
Ma JC;Wu YQ;Cao D;Zhang WB;Wang HH
铜绿假单胞菌基因组含有3个开放阅读框PA2966、PA1869和PA3334，分别编码AcpP1、AcpP2和AcpP3。在本研究中，我们发现，虽然这些apo-ACPs被铜绿假单胞菌磷酸丙氨酸基转移酶(PCPS)成功地磷酸化，并且这些蛋白的所有全息形式都可以被哈维氏弧菌酰基-ACP合成酶(AASS)酰化，但只有AcpP1在体外可以作为底物在铜绿假单胞菌无细胞提取物的催化下合成脂肪酸，并且只有acpP1基因能够恢复在大肠杆菌acpp突变株CY1877中的生长。而铜绿假单胞菌acpP1不能被缺失，而突变株acpP2或acpP3的缺失使突变株与野生型菌株一样生长。这些发现证实，只有铜绿假单胞菌AcpP1在脂肪酸生物合成中起作用，而acpP2和acpP3在脂肪酸合成途径中不起作用。此外，acpP2和acpP3的干扰不影响铜绿假单胞菌产生N-酰基高丝氨酸内酯(AHL)的能力，但用大肠杆菌acpP替换acpP1导致铜绿假单胞菌AHL分子的产生减少，这表明无论是AcpP2还是AcpP3都不能在体内作为合成AHL分子的底物。此外，用大肠杆菌acpP替换acpP1降低了铜绿假单胞菌产生某些外源产物的能力，并取消了铜绿假单胞菌的群体运动。
The genome of Pseudomonas aeruginosa contains three open reading frames, PA2966, PA1869, and PA3334, which encode putative acyl carrier proteins, AcpP1, AcpP2, and AcpP3, respectively. In this study, we found that, although these apo-ACPs were successfully phosphopantetheinylated by P. aeruginosa phosphopantetheinyl transferase (PcpS) and all holo-forms of these proteins could be acylated by Vibrio harveyi acyl-ACP synthetase (AasS), only AcpP1 could be used as a substrate for the synthesis of fatty acids, catalyzed by P. aeruginosa cell free extracts in vitro, and only acpP1 gene could restore growth in the Escherichia coli acpP mutant strain CY1877. And P. aeruginosa acpP1 could not be deleted, while disruption of acpP2 or acpP3 in the P. aeruginosa genome allowed mutant strains to grow as well as the wild type strain. These findings confirmed that only P. aeruginosa AcpP1 functions in fatty acid biosynthesis, and that acpP2 and acpP3 do not play roles in the fatty acid synthetic pathway. Moreover, disruption of acpP2 and acpP3 did not affect the ability of P. aeruginosa to produce N-acylhomoserine lactones (AHL), but replacement of P. aeruginosa acpP1 with E. coli acpP caused P. aeruginosa to reduce the production of AHL molecules, which indicated that neither P. aeruginosa AcpP2 nor AcpP3 can act as a substrate for synthesis of AHL molecules in vivo. Furthermore, replacement of acpP1 with E. coli acpP reduced the ability of P. aeruginosa to produce some exo-products and abolished swarming motility in P. aeruginosa.
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影响因子：
3.7
作者：
Mao YH;Ma JC;Li F;Hu Z;Wang HH
通讯作者：
Wang HH
影响因子：
3.2
作者：
Hoang, TT;Schweizer, HP
通讯作者：
Schweizer, HP
影响因子：
3.2
作者：
ESSAR, DW;EBERLY, L;CRAWFORD, IP
通讯作者：
CRAWFORD, IP
DOI：
10.1099/00221287-143-12-3703
发表时间：
1997-12-01
期刊：
MICROBIOLOGY-UK
影响因子：
--
作者：
McClean, KH;Winson, MK;Williams, P
通讯作者：
Williams, P
影响因子：
3.5
作者：
Hannauer, Melissa;Schaefer, Mathias;Schalk, Isabelle J.
通讯作者：
Schalk, Isabelle J.