High‐level expression of active HIV‐1 integrase from a synthetic gene in human cells
High‐level expression of active HIV‐1 integrase from a synthetic gene in human cells
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人类细胞中合成基因的活性 HIV-1 整合酶的高水平表达
DOI：
10.1096/fasebj.14.10.1389
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发表时间：
2000
期刊：
影响因子：
--
通讯作者：
Z. Debyser
中科院分区：
文献类型：
--
作者：
P. Cherepanov;W. Pluymers;Anje Claeys;P. Proost;E. Clercq;Z. Debyser
设计了一种编码HIV - 1整合酶的合成基因，以规避野生型基因中存在的固有不稳定性和抑制因子。在多种独立于病毒附属蛋白的人类细胞系中获得了HIV - 1整合酶的高水平表达。我们选择了一个稳定表达HIV‐1整合酶的人293T细胞系，其生长动力学与亲本细胞系相当。该酶定位于细胞核，并在有丝分裂期间稳定地与染色体相关。携带失活整合酶基因D64V突变的慢病毒载体颗粒在合成基因表达的整合酶的产生细胞中补充后，能够稳定地转导293T细胞。当稳定表达整合酶的细胞系被缺陷病毒颗粒感染时，也检测到整合酶活性的互补。在人类细胞中表达活跃的HIV‐1整合酶将有助于研究宿主和病毒因子在整合过程中的相互作用。-Cherepanov， P., Pluymers， W., Claeys， A., Proost， P., De Clercq， E., Debyser， Z.人类细胞中HIV - 1整合酶的高水平表达。中华医学杂志，14 (2),389 - 399 （2000）
A synthetic gene encoding for HIV‐1 integrase was designed to circumvent the intrinsic instability and the repressor elements present in the wild‐type gene. High‐level expression of HIV‐1 integrase was obtained in various human cell lines independently of viral accessory proteins. A human 293T cell line was selected that stably expresses HIV‐1 integrase and has growth kinetics comparable to the parental cell line. The enzyme was localized in the nucleus and remained stably associated with the chromosomes during mitosis. Lentiviral vector particles carrying the inactivating D64V mutation in the integrase gene were capable of stably transducing 293T cells when complemented in the producer cells with integrase expressed from the synthetic gene. When the cell line that stably expresses integrase was infected with the defective viral particles, complementation of integrase activity was detected as well. Expression of active HIV‐1 integrase in human cells will facilitate the study of the interplay between host and viral factors during integration.—Cherepanov, P., Pluymers, W., Claeys, A., Proost, P., De Clercq, E., Debyser, Z. High‐level expression of active HIV‐1 integrase from a synthetic gene in human cells. FASEB J. 14, 1389–1399 (2000)
影响因子：
56.9
作者：
KALPANA, GV;MARMON, S;GOFF, SP
通讯作者：
GOFF, SP
表达劳斯肉瘤病毒整合酶的稳定真核细胞系的表征。
影响因子：
3.7
作者：
Mumm,SR;Hippenmeyer,PJ;Grandgenett,DP
通讯作者：
Grandgenett,DP
影响因子：
56.9
作者：
Daniel, R;Katz, RA;Skalka, AM
通讯作者：
Skalka, AM