Exhaled breath analysis using electronic nose in cystic fibrosis and primary ciliary dyskinesia patients with chronic pulmonary infections.
Exhaled breath analysis using electronic nose in cystic fibrosis and primary ciliary dyskinesia patients with chronic pulmonary infections.
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DOI：
10.1371/journal.pone.0115584
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Nielsen KG
中科院分区：
文献类型：
--
作者：
Joensen O;Paff T;Haarman EG;Skovgaard IM;Jensen PØ;Bjarnsholt T;Nielsen KG
目前肺部感染的诊断检查和监测可能被认为是侵入性的，耗时且昂贵。在这项探索性研究中，我们研究了使用电子鼻的非侵入性呼出气分析是否可以区分囊性纤维化（CF）和原发性纤毛运动障碍（PCD），伴或不伴各种特征明确的慢性肺部感染。我们招募了64名CF患者和21名PCD患者，这些患者均基于已知的慢性感染状态。21例健康志愿者作为对照组。采用电子鼻分析呼出气样品。主成分减少和判别分析被用来构建内部交叉验证的受试者工作特征（ROC）曲线。CF和PCD患者的呼吸曲线分别与健康对照组显著不同，p = 0.001和p = 0.005。 患有慢性铜绿假单胞菌感染的CF患者的特征与非慢性感染的CF患者显著不同，p = 0.044。 我们证实了先前建立的呼出气分析在健康受试者和CF或PCD患者之间的区分能力。此外，该方法显著区分患有慢性肺部铜绿假单胞菌（PA）感染的CF患者与没有慢性肺部感染的CF患者。需要进一步的研究来验证和调查电子鼻技术在建立慢性感染之前对肺部感染的早期诊断中的作用。
The current diagnostic work-up and monitoring of pulmonary infections may be perceived as invasive, is time consuming and expensive. In this explorative study, we investigated whether or not a non-invasive exhaled breath analysis using an electronic nose would discriminate between cystic fibrosis (CF) and primary ciliary dyskinesia (PCD) with or without various well characterized chronic pulmonary infections. We recruited 64 patients with CF and 21 with PCD based on known chronic infection status. 21 healthy volunteers served as controls. An electronic nose was employed to analyze exhaled breath samples. Principal component reduction and discriminant analysis were used to construct internally cross-validated receiver operator characteristic (ROC) curves. Breath profiles of CF and PCD patients differed significantly from healthy controls p = 0.001 and p = 0.005, respectively. Profiles of CF patients having a chronic P. aeruginosa infection differed significantly from to non-chronically infected CF patients p = 0.044. We confirmed the previously established discriminative power of exhaled breath analysis in separation between healthy subjects and patients with CF or PCD. Furthermore, this method significantly discriminates CF patients suffering from a chronic pulmonary P. aeruginosa (PA) infection from CF patients without a chronic pulmonary infection. Further studies are needed for verification and to investigate the role of electronic nose technology in the very early diagnostic workup of pulmonary infections before the establishment of a chronic infection.
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影响因子：
6.7
作者：
Bos LD;Sterk PJ;Schultz MJ
通讯作者：
Schultz MJ
影响因子：
9.4
作者：
de Heer, Koen;van der Schee, Marc P.;Sterk, Peter J.
通讯作者：
Sterk, Peter J.
影响因子：
35
作者：
Dam, Jeppe Seidelin;Tidemand-Lichtenberg, Peter;Pedersen, Christian
通讯作者：
Pedersen, Christian
DOI：
10.1183/1025448x.00018509
发表时间：
2010-01-01
期刊：
EXHALED BIOMARKERS
影响因子：
--
作者：
Amann, A.;Miekisch, W.;Schubert, J.
通讯作者：
Schubert, J.
影响因子：
3.8
作者：
Gilchrist, Francis J.;Bright-Thomas, Rowland J.;Lenney, Warren
通讯作者：
Lenney, Warren