Characteristic comparison of triglyceride-rich remnant lipoprotein measurement between a new homogenous assay (RemL-C) and a conventional immunoseparation method (RLP-C)
Characteristic comparison of triglyceride-rich remnant lipoprotein measurement between a new homogenous assay (RemL-C) and a conventional immunoseparation method (RLP-C)
复制标题
新型均质测定法 (RemL-C) 与传统免疫分离方法 (RLP-C) 测量富含甘油三酯的残余脂蛋白的特性比较
作者：
Hiroshi Yoshida;Hideo Kurosawa;Y. Hirowatari;Yutaka Ogura;Katsunori Ikewaki;I. Abe;Shinichi Saikawa;Kenichi Domitsu;Kumie Ito;H. Yanai;N. Tada
背景：除了LDL升高外，血清残余脂蛋白升高被认为可以预测心血管疾病。残馀脂蛋白胆固醇（RLP-C）是一种广泛用于残馀脂蛋白胆固醇测定的免疫分离法，目前已开发出一种新的残馀脂蛋白胆固醇（RemL-C）均相测定法。方法对83例无糖尿病、高血压、无高脂血症、糖尿病、高血压药物的受试者（男49例，女34例）进行相关性分析和数据验证，探讨两种方法（RLP-C和RemL-C）所得残余脂蛋白的特征及其与HPLC法测定的idl -胆固醇的关系。结果两种方法之间存在显著正相关（r = 0.853, 95%CI 0.781 ~ 0.903, p < 0.0001）。Bland & Altman分析显示，RemL-C值可能显著高于RLP-C值，特别是在残余脂蛋白水平较高的样品中。还观察到RemL-C和RLP-C之间的一些数据分离。HPLC图谱显示，RemL-C水平< RLP-C水平的血清中乳糜微粒胆固醇浓度较高，而RemL-C水平< RLP-C水平的血清中idl -胆固醇浓度较高。所有样本（n = 83） RemL-C （r = 0.339, 95%CI 0.152 ~ 0.903; p = 0.0005）与idl -胆固醇显著相关，RLP-C （r = 0.17, 95%CI -0.047 ~ 0.372; p = 0.1237）无显著相关性。结论RemL-C与RLP-C之间普遍存在显著相关性。然而，RemL-C分析可能比RLP-C更能反映IDL。
BackgroundIncreased serum remnant lipoproteins are supposed to predict cardiovascular disease in addition to increased LDL. A new homogenous assay for remnant lipoprotein-cholesterol (RemL-C) has been developed as an alternative to remnant-like particle-cholesterol (RLP-C), an immunoseparation assay, widely used for the measurement of remnant lipoprotein cholesterol.MethodsWe evaluated the correlations and data validation between the 2 assays in 83 subjects (49 men and 34 women) without diabetes, hypertension and medications for hyperlipidemia, diabetes, and hypertension, and investigated the characteristics of remnant lipoproteins obtained by the two methods (RLP-C and RemL-C) and their relationships with IDL-cholesterol determined by our developed HPLC method.ResultsA positive correlation was significantly found between the two methods (r = 0.853, 95%CI 0.781–0.903, p < 0.0001). Bland & Altman analysis revealed that RemL-C values were likely to be significantly higher than RLP-C values, particularly in samples with high levels of remnant lipoproteins. Several data dissociations between the RemL-C and RLP-C were also observed. The HPLC chromatograms show high concentrations of chylomicron cholesterol in serum samples with RemL-C level < RLP-C level, but high concentrations of IDL-cholesterol in samples with RemL-C level > RLP-C level. RemL-C (r = 0.339, 95%CI 0.152–0.903; p = 0.0005) significantly correlated with IDL-cholesterol, but not RLP-C (r = 0.17, 95%CI -0.047–0.372; p = 0.1237) in all the samples (n = 83).ConclusionThese results suggest that there is generally a significant correlation between RemL-C and RLP-C. However, RemL-C assay is likely to reflect IDL more closely than RLP-C.
影响因子：
6.5
作者：
F. Karpe;S. Boquist;Rong Tang;Gene M. Bond;U. Faire;Anders Hamsten
通讯作者：
F. Karpe;S. Boquist;Rong Tang;Gene M. Bond;U. Faire;Anders Hamsten