Poliovirus temperature-sensitive mutant containing a single nucleotide deletion in the 5'-noncoding region of the viral RNA.
Poliovirus temperature-sensitive mutant containing a single nucleotide deletion in the 5'-noncoding region of the viral RNA.
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脊髓灰质炎病毒温度敏感突变体，在病毒 RNA 的 5-非编码区含有单核苷酸缺失。
DOI：
10.1016/0042-6822(86)90211-4
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发表时间：
1986
期刊：
影响因子：
3.7
通讯作者：
Meriam,C
中科院分区：
文献类型：
--
作者：
Racaniello,VR;Meriam,C
确定了删除脊髓灰质炎病毒RNA基因组第10位核苷酸对病毒复制的影响。这种删除从病毒 RNA 中预测的发夹结构中删除了一个碱基对，并被引入全长 cDNA 中。用包含预期缺失的突变 cDNA 转染 HeLa 细胞后，病毒恢复，并且对噬菌斑形成温度敏感。通过一步生长实验对病毒复制进行分析表明，在非允许温度下突变病毒的产量至少比野生型病毒少100倍，并且病毒从突变感染细胞中的释放被延迟。突变病毒感染细胞中正链和负链病毒RNA的合成对温度敏感。突变病毒感染细胞中的病毒特异性蛋白质合成对温度不敏感，但在允许和不允许的温度下，其发生速度比野生型病毒慢。突变病毒的复制对放线菌素 D 敏感，而野生型亲本病毒对该药物具有抗性。突变病毒库存含有一小部分 ts+ 病毒，这些病毒能够在不允许的温度下形成噬斑。对这些 ts+ 病毒基因组 RNA 的核苷酸序列分析显示，第 34 位的单碱基从 G 变为 U。在正 RNA 链中，这种突变的作用是将因原始删除而阻止形成的单碱基对恢复到发夹结构。 ts+假回复体在 33 和 38.5° 复制到与野生型病毒相似的滴度，并且对放线菌素 D 部分敏感。
The effect on viral replication of deleting nucleotide 10 of the poliovirus RNA genome was determined. This deletion, which removes a base pair from a predicted hairpin structure in the viral RNA, was introduced into full-length cDNA. Virus recovered after transfection of HeLa cells with the mutated cDNA contained the expected deletion and was temperature sensitive for plaque formation. Analysis of viral replication by one-step growth experiments indicated that mutant virus production at the nonpermissive temperature was at least 100 times less than that of wild type virus, and release of virus from mutant-infected cells was delayed. The synthesis of positive- and negative-strand viral RNA in mutant virus-infected cells was temperature sensitive. Virus-specific protein synthesis in mutant virus-infected cells was not temperature sensitive but occurred at a slower rate than that of wild type virus at permissive and nonpermissive temperatures. Replication of the mutant virus was sensitive to actinomycin D, in contrast to the wild type parent virus, which was resistant to the drug. Mutant virus stocks contained a small percentage of ts+viruses that were able to form plaques at the nonpermissive temperature. Nucleotide sequence analysis of genomic RNA from these ts+viruses revealed a single base change at position 34 from a G to U. In the positive RNA strand, the effect of this mutation is to restore to the hairpin structure the single base pair whose formation was prevented by the original deletion. The ts+pseudorevertants replicated to similar titers as wild type virus at 33 and 38.5° and were partially sensitive to actinomycin D.
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氯化镁增强细胞对脊髓灰质炎病毒的易感性。
影响因子：
3.7
作者：
C. Wallis;J. Melnick
通讯作者：
J. Melnick
放线菌素 D 对脊髓灰质炎病毒增殖 HEp 2 细胞的抑制。
影响因子：
3.7
作者：
C. Grado;S. Fischer;G. Contreras
通讯作者：
G. Contreras
脊髓灰质炎病毒 RNA 的合成和病毒颗粒的形成方面。
影响因子：
3.7
作者：
D. Baltimore;M. Girard;J. Darnell
通讯作者：
J. Darnell
来自脊髓灰质炎病毒和柯萨奇病毒的 cDNA 克隆的嵌合质粒产生了对温度敏感的重组病毒。
DOI：
10.1073/pnas.83.6.1777
发表时间：
1986
影响因子：
11.1
作者：
Semler,BL;Johnson,VH;Tracy,S
通讯作者：
Tracy,S
放线菌素 D 对脊髓灰质炎病毒株的不同抑制作用
影响因子：
3.2
作者：
F. L. Schaffer;M. Gordon
通讯作者：
M. Gordon