Densitometry determination of oestrogenic EDCs with gold nanoparticle-modified oestrogen response element assay
Densitometry determination of oestrogenic EDCs with gold nanoparticle-modified oestrogen response element assay
复制标题
用金纳米颗粒修饰的雌激素反应元件测定光密度法测定雌激素 EDC
DOI：
10.1080/03067310903199518
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发表时间：
2011-03
期刊：
影响因子：
--
通讯作者：
Shun-qing Xu
中科院分区：
文献类型：
--
作者：
Tian Chen;Yan-jian Wan;Yu-Qiu Gao;Yuan-yuan Li;Shun-qing Xu
雌激素受体结合测定是筛选雌激素内分泌干扰物的重要方法。但往往价格昂贵，并且存在放射性污染。为了在没有放射性标记的情况下筛选内分泌干扰化学物质（EDC），我们开发了一种利用金纳米颗粒技术的新高通量方法。该测定基于样品中雌激素 EDC 和 17β-雌二醇-BSA 与雌激素受体之间的竞争结合。信号来自标记 ERE 的金纳米颗粒与配体-受体复合物的特异性结合。结果表明，可检测出低至 100 pg L−1 的 17β-雌二醇，线性范围为 100 pg L−1 至 1 μg L−1 (R 2 = 0.9764)。通过我们的方法和细胞 (MCF-7) 增殖测定的环境样品中雌激素 EDC 的浓度没有显着差异。结果表明，该方法是一种理想的筛选方法，可靠、低成本、快速、高通量，并且可以在微孔板或芯片上进行。
Oestrogen receptor binding assay is an important approach to screen oestrogenic endocrine disruptors. But it is often expensive and radioactive pollution has existed. In order to screening endocrine disrupting chemicals (EDCs) without a radioactive label, we developed a new high-throughout method using gold nanoparticle technology. The assay is based on the competition binding between the oestrogenic EDCs in the sample and 17β-estradiol-BSA to the oestrogen receptor. The signal is from specific binding of gold nanoparticles labelled ERE to the ligand-receptor complex. The result showed that as little as 100 pg L−1 of 17β-estradiol could be detected with a linear range from 100 pg L−1 to 1 µg L−1 (R 2 = 0.9764). The concentrations of oestrogenic EDCs in environmental sample determined by our method and by the cell (MCF-7) proliferation were not significantly different. The result presented led us to conclude that this method is an ideal screening method which is reliable, low-cost, rapid, high-throughout and could be performed on microplates or chips.
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