Effects of Levonorgestrel and progesterone on Oviductal physiology in mammals.
Effects of Levonorgestrel and progesterone on Oviductal physiology in mammals.
复制标题
左炔诺孕酮和黄体酮对哺乳动物输卵管生理的影响
DOI：
10.1186/s12958-018-0377-3
复制
发表时间：
2018-06-20
期刊：
影响因子：
--
通讯作者：
Zhang J
中科院分区：
文献类型：
--
作者：
Li C;Zhang HY;Liang Y;Xia W;Zhu Q;Zhang D;Huang Z;Liang GL;Xue RH;Qi H;He XQ;Yuan JJ;Tan YJ;Huang HF;Zhang J
背景我们之前的研究表明，紧急避孕药，包括左炔诺孕酮和黄体酮，可能会在避孕失败后导致宫外孕。然而，我们对左炔诺孕酮和黄体酮对输卵管生理学影响的了解是有限的。方法通过接受性标记物的蛋白质印迹和JAr球体-输卵管上皮细胞附着试验检查左炔诺孕酮和黄体酮治疗后输卵管上皮的接受性。使用倒置明视野显微镜分析纤毛跳动频率。此外，还研究了胚胎输卵管移植的体内动物模型，以确定左炔诺孕酮和黄体酮诱导的纤毛跳动减少的效果。结果我们的结果表明，左炔诺孕酮和黄体酮不会改变输卵管上皮细胞接受标志物的水平，包括LIF、STAT3、IGFBP1、ITGB3、MUC1和ACVR1B，也不会影响JAr球体植入。然而，左炔诺孕酮和黄体酮以剂量依赖性方式降低输卵管纤毛跳动频率。体内模型还表明，左炔诺孕酮和黄体酮可以导致胚胎滞留在输卵管中。结论这些发现表明，左炔诺孕酮和黄体酮可以降低纤毛跳动频率而不改变容受性，这表明黄体酮或左炔诺孕酮诱导输卵管妊娠的可能机制。
BackgroundOur previous study indicated that emergency contraception, including levonorgestrel and progesterone, could lead to ectopic pregnancy following contraception failure. However, our understanding of the effects of levonorgestrel and progesterone on oviductal physiology is limited.MethodsThe receptivity of the fallopian tubal epithelium after levonorgestrel and progesterone treatment was examined through western blots for receptivity markers and JAr-spheroid-fallopian tubal epithelial cell attachment assays. The ciliary beat frequency was analyzed using an inverted bright-field microscope. Furthermore, an in vivo animal model of embryo-tubal transplantation was also studied to determine the effects of levonorgestrel- and progesterone-induced ciliary beat reduction.ResultsOur results showed that levonorgestrel and progesterone did not change the levels of fallopian tubal epithelial cell receptive markers, including LIF, STAT3, IGFBP1, ITGB3, MUC1, and ACVR1B, or affect JAr-spheroid implantation. However, levonorgestrel and progesterone reduced the ciliary beat frequency in fallopian tubes in a dose-dependent manner. An in vivo model also showed that levonorgestrel and progesterone could lead to embryo retention in the oviducts.ConclusionsThese findings show that levonorgestrel and progesterone can reduce the ciliary beat frequency without altering receptivity, indicating a possible mechanism for progesterone- or levonorgestrel-induced tubal pregnancy.
登录
查看更多内容
影响因子：
2.9
作者：
Ghosh, Bhaswati;Dadhwal, Vatsla;Mittal, Suneeta
通讯作者：
Mittal, Suneeta
影响因子：
2.5
作者：
Lee, YL;Lee, KF;Yeung, WSB
通讯作者：
Yeung, WSB
DOI：
10.1038/labinvest.2011.148
发表时间：
2012-02
期刊：
Laboratory investigation; a journal of technical methods and pathology
影响因子：
--
作者：
通讯作者：
--
影响因子：
3.1
作者：
Li C;Zhao WH;Zhu Q;Cao SJ;Ping H;Xi X;Qin GJ;Yan MX;Zhang D;Qiu J;Zhang J
通讯作者：
Zhang J
影响因子：
3.6
作者：
HALBERT, SA;BECKER, DR;SZAL, SE
通讯作者：
SZAL, SE