The Ca2+/calcineurin-dependent signaling pathway in the gray mold Botrytis cinerea: the role of calcipressin in modulating calcineurin activity.
The Ca2+/calcineurin-dependent signaling pathway in the gray mold Botrytis cinerea: the role of calcipressin in modulating calcineurin activity.
复制标题
DOI：
10.1371/journal.pone.0041761
复制
发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Tudzynski B
中科院分区：
文献类型：
--
作者：
Harren K;Schumacher J;Tudzynski B
在灰霉菌Botrytis cinerea中，异三聚体G蛋白的Gα亚基Bcg1是钙/钙调素依赖的磷酸酶钙调神经磷酸酶的上游激活剂。在这项研究中，我们重点研究了钙调神经磷酸酶催化亚单位(BcCnA)及其可能的调节因子降钙素(BcRcn1)的功能特性。我们删除了编码这两种蛋白的基因，以检查它们在生长、分化和毒力方面的作用。Δ的bccnA突变体表现出严重的生长缺陷，不产生分生孢子，无毒，而BcRcn1的缺失导致了菌丝生长迟缓和寄主植物的延迟侵染，但对分生孢子和菌核的形成没有影响。BcRcn1对几个钙调神经磷酸酶依赖基因和bccnA本身的表达有正向影响。将Δ突变体与GFP-BcRcn1融合构建体互补，发现BcRcn1定位于细胞质中，并聚集在细胞核周围。此外，我们还发现BcCnA与BcRcn1和钙调神经磷酸酶的调节亚基BcCnB有物理上的相互作用。我们通过研究BcCnA与野生型和突变的BcRcn1之间的物理相互作用，研究了BcRcn1调节和激活BcCnA的几个蛋白质结构域特征的影响，如磷酸化位点和钙调神经磷酸酶对接位点。根据观察到的表型，我们得出结论：BcRcn1是BcCnA和钙/钙调神经磷酸酶介导的信号转导的正向调节器，这两种蛋白都调节真菌的发育和毒力。
In the gray mold fungus Botrytis cinerea the Gα subunit Bcg1 of a heterotrimeric G protein is an upstream activator of the Ca2+/calmodulin-dependent phosphatase calcineurin. In this study we focused on the functional characterization of the catalytic subunit of calcineurin (BcCnA) and its putative regulator calcipressin (BcRcn1). We deleted the genes encoding both proteins to examine their role concerning growth, differentiation and virulence. The ΔbccnA mutant shows a severe growth defect, does not produce conidia and is avirulent, while the loss of BcRcn1 caused retardation of hyphal growth and delayed infection of host plants, but had no impact on conidiation and sclerotia formation. Expression of several calcineurin-dependent genes and bccnA itself is positively affected by BcRcn1. Complementation of the Δbcrcn1 mutant with a GFP-BcRcn1 fusion construct revealed that BcRcn1 is localized in the cytoplasm and accumulates around the nuclei. Furthermore, we showed that BcCnA physically interacts with BcRcn1 and the regulatory subunit of calcineurin, BcCnB. We investigated the impact of several protein domains characteristic for modulation and activation of BcCnA via BcRcn1, such as the phosphorylation sites and the calcineurin-docking site, by physical interaction studies between BcCnA and wild-type and mutated copies of BcRcn1. Based on the observed phenotypes we conclude that BcRcn1 acts as a positive modulator of BcCnA and the Ca2+/calcineurin-mediated signal transduction in B. cinerea, and that both proteins regulate fungal development and virulence.
登录
查看更多内容
影响因子：
16
作者：
Aramburu, J;Garcia-Cozar, F;Hogan, PG
通讯作者：
Hogan, PG
影响因子：
2.5
作者：
Hagiwara, Daisuke;Kondo, Atsushi;Abe, Keietsu
通讯作者：
Abe, Keietsu
影响因子：
3.5
作者：
Gronover, CS;Kasulke, D;Tudzynski, B
通讯作者：
Tudzynski, B
影响因子：
11.4
作者：
Görlach, J;Fox, DS;Heitman, J
通讯作者：
Heitman, J
影响因子：
3.1
作者：
Bader, Teresa;Schroeppel, Klaus;Morschhaeuser, Joachim
通讯作者：
Morschhaeuser, Joachim