Identification of specific relaxin-binding cells in the cervix, mammary glands, nipples, small intestine, and skin of pregnant pigs.
Identification of specific relaxin-binding cells in the cervix, mammary glands, nipples, small intestine, and skin of pregnant pigs.
复制标题
鉴定怀孕猪的子宫颈、乳腺、乳头、小肠和皮肤中的特异性松弛素结合细胞。
作者：
Gyesik Min;O. Sherwood
我们之前已经证明松弛素可以促进怀孕猪子宫颈的生长和软化以及乳腺的发育。了解松弛素在这些组织中的作用机制的一个重要方面是识别含有松弛素受体的特定细胞类型，即识别那些启动松弛素作用的细胞。本研究的目的是鉴定怀孕猪体内已知对松弛素有反应的组织（子宫颈和乳腺）以及疑似对松弛素有反应的组织（乳头、小肠和皮肤）中的松弛素结合细胞。为了实现这一目标，我们开发了一种体外修饰的免疫组织化学技术，该技术最近用于鉴定松弛素结合细胞。采用两组妊娠后备母猪：完整对照组（C组）和去卵巢孕激素治疗组（OP组）。OP组于妊娠第40天（第40天）去卵巢，给予孕酮（50 mg/2 ml玉米油灌胃，每日2次）至第110天维持妊娠。在第110天，从两组中取出组织，切成立方体（2-3 cm3），在液氮中冷冻，并冷冻切片（8微米）。结合松弛素的特定细胞类型通过连续应用生物素化松弛素探针、抗生素素免疫球蛋白G偶联到1 nm胶体金和用于信号放大的银来鉴定。该研究首次证明了松弛素特异性地与1)血管（宫颈、乳腺、乳头、小肠）结合；2)小肠平滑肌（环状、纵向、肌层粘膜）；3)乳房乳头以外部位的皮肤（背部、耳朵、大腿、腿部）。此外，与先前在大鼠中的发现一致，在子宫颈、乳腺和乳头的上皮细胞中观察到显著的标记；在宫颈和乳头的平滑肌细胞中；还有乳头的皮肤。在松弛素结合方面，C组和op组没有明显差异。我们推断，怀孕猪的子宫颈、乳腺、乳头、小肠和皮肤中的特定松弛素结合细胞可能含有松弛素受体，因此介导了松弛素在这些组织中的作用。
We previously demonstrated that relaxin promotes growth and softening of the cervix and development of the mammary glands in the pregnant pig. An important aspect of understanding relaxin's mechanism of action in these tissues is to identify the specific cell type(s) that contains relaxin receptors, that is, to identify those cells that initiate relaxin's effects. The objective of the present study was to identify relaxin-binding cells in tissues known to respond to relaxin (cervix and mammary gland) as well as in tissues suspected of being responsive to relaxin (nipple, small intestine, and skin) in the pregnant pig. To accomplish that objective we developed an in vitro modification of an immunohistochemical technique recently developed for identification of relaxin-binding cells. Two groups of pregnant gilts were used: intact control (group C) and ovariectomized progesterone-treated (group OP). Group OP was ovariectomized on Day 40 of gestation (Day 40) and treated with progesterone (50 mg/2 ml corn oil i.m., twice daily) until Day 110 to maintain pregnancy. On Day 110, tissues from both groups were removed, cut into cubes (2-3 cm3), frozen in liquid nitrogen, and cryosectioned (8 microns). Specific cell types that bind relaxin were identified by sequential application of a biotinylated relaxin probe, antibiotin immunoglobulin G conjugated to 1 nm colloidal gold, and silver for signal amplification. The study demonstrates for the first time that relaxin binds with specificity to 1) blood vessels (cervix, mammary glands, nipples, small intestine); 2) smooth muscles in small intestine (circular, longitudinal, muscularis mucosa); and 3) skin from sites other than the mammary nipples (back, ear, thigh, leg). In addition, consistent with previous findings in the rat, prominent labeling was observed in epithelial cells in the cervix, mammary glands, and nipples; in smooth muscle cells in the cervix and mammary nipples; and in the skin of the nipples. There were no apparent differences in relaxin binding between group C and group OP. We conclude that the specific relaxin-binding cells in the cervix, mammary glands, nipples, small intestine, and skin of the pregnant pig probably contain relaxin receptors and, therefore, mediate relaxin's effects in these tissues.
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影响因子：
4.8
作者：
HURLEY, WL;DOANE, RM;SHERWOOD, OD
通讯作者：
SHERWOOD, OD
松弛素、雌激素和黄体酮对去卵巢后备母猪的单独和联合作用。
影响因子：
4.8
作者：
Winn,RJ;Baker,MD;Sherwood,OD
通讯作者：
Sherwood,OD
DOI：
10.1530/jrf.0.0660359
发表时间：
1982
期刊：
Journal of reproduction and fertility
影响因子：
--
作者：
Nara,BS;Welk,FA;Rutherford,JE;Sherwood,OD;First,NL
通讯作者：
First,NL
影响因子：
4.8
作者：
R. Winn;M. Baker;C. Merle;O. Sherwood
通讯作者：
R. Winn;M. Baker;C. Merle;O. Sherwood
影响因子：
4.8
作者：
Kuenzi,MJ;Sherwood,OD
通讯作者：
Sherwood,OD