Green synthesis of silver nanoparticles through reduction with Solanum xanthocarpum L. berry extract: characterization, antimicrobial and urease inhibitory activities against Helicobacter pylori.
Green synthesis of silver nanoparticles through reduction with Solanum xanthocarpum L. berry extract: characterization, antimicrobial and urease inhibitory activities against Helicobacter pylori.
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DOI：
10.3390/ijms13089923
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发表时间：
2012
影响因子：
5.6
通讯作者：
Rashid U
中科院分区：
文献类型：
--
作者：
Amin M;Anwar F;Janjua MRSA;Iqbal MA;Rashid U
本文报道了以黄果茄为原料，甲醇提取物制备纳米银的绿色合成路线。用SXE（盖层和还原剂）与AgNO3在45℃下反应25 min，合成了以406 nm为中心的表面等离子体共振（SPR）带的银纳米粒子（AgNps）。采用紫外可见分光光度法、粉末x射线衍射法和透射电镜（TEM）对合成的AgNps进行了表征。结果表明：反应时间、温度和SXE与AgNO3的体积比均能加快Ag+的还原速率，影响AgNps的尺寸和形状；纳米颗粒的尺寸约为10纳米，性质为单分散，形状为球形。采用琼脂稀释法测定合成的AgNps对34株临床分离株和2株幽门螺杆菌的体外抗幽门螺杆菌活性，并与AgNO3及抗幽门螺杆菌用标准药物阿莫西林（AMX）、克拉霉素（CLA）、甲硝唑（MNZ）和四环素（TET）进行比较。螺杆菌疗法。典型AgNps样品（S1）能有效抑制幽门螺杆菌的生长，表明其抗幽门螺杆菌活性较强。与AgNO3或MNZ相比，其对TET的作用几乎相同，但低于AMX和CLA。研究中发现AgNps对抗生素耐药和抗生素敏感的幽门螺杆菌菌株同样有效。此外，在幽门螺杆菌脲酶抑制实验中，S1也表现出明显的抑制作用。linewever - burk图显示抑制机制是非竞争性的。
A green synthesis route for the production of silver nanoparticles using methanol extract from Solanum xanthocarpum berry (SXE) is reported in the present investigation. Silver nanoparticles (AgNps), having a surface plasmon resonance (SPR) band centered at 406 nm, were synthesized by reacting SXE (as capping as well as reducing agent) with AgNO3 during a 25 min process at 45 °C. The synthesized AgNps were characterized using UV–Visible spectrophotometry, powdered X-ray diffraction, and transmission electron microscopy (TEM). The results showed that the time of reaction, temperature and volume ratio of SXE to AgNO3 could accelerate the reduction rate of Ag+ and affect the AgNps size and shape. The nanoparticles were found to be about 10 nm in size, mono-dispersed in nature, and spherical in shape. In vitro anti-Helicobacter pylori activity of synthesized AgNps was tested against 34 clinical isolates and two reference strains of Helicobacter pylori by the agar dilution method and compared with AgNO3 and four standard drugs, namely amoxicillin (AMX), clarithromycin (CLA), metronidazole (MNZ) and tetracycline (TET), being used in anti-H. pylori therapy. Typical AgNps sample (S1) effectively inhibited the growth of H. pylori, indicating a stronger anti-H. pylori activity than that of AgNO3 or MNZ, being almost equally potent to TET and less potent than AMX and CLA. AgNps under study were found to be equally efficient against the antibiotic-resistant and antibiotic-susceptible strains of H. pylori. Besides, in the H. pylori urease inhibitory assay, S1 also exhibited a significant inhibition. Lineweaver-Burk plots revealed that the mechanism of inhibition was noncompetitive.
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影响因子：
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作者：
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影响因子：
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