Clock genes and diurnal transcriptome dynamics in summer and winter in the gymnosperm Japanese cedar (Cryptomeria japonica (L.f.) D.Don)
Clock genes and diurnal transcriptome dynamics in summer and winter in the gymnosperm Japanese cedar (Cryptomeria japonica (L.f.) D.Don)
复制标题
裸子植物日本雪松 (Cryptomeria japonica (L.f.) D.Don) 夏季和冬季的时钟基因和昼夜转录组动态
作者：
M. Nose;A. Watanabe
背景转录组的生物钟和昼夜动态被认为在调节与植物环境的昼夜和年度周期同步的生理、生物和发育过程中发挥着重要作用。然而，人们对裸子植物的生物钟及其调节知之甚少，包括针叶树。本文研究了日本雪松(Cryptomeria Japan onica(L.F.)结果基因芯片分析表明，夏季和冬季的转录本存在显著差异，只有夏季才有转录组的昼夜动态。芯片上约7.7%的独特基因(7254个中的556个)在夏季周期性表达。一些基因的表达模式，特别是与光相关的基因，在日本雪松中没有表现出显著的振荡，因此与被子植物中报道的不同。基因网络分析揭示了与可能的核心时钟基因(CjLHYa、CjLHYb、CjTOC1、CjGI和CjZTL)相关的网络，这表明它们在转录的日调节中具有重要作用。结论本研究揭示了雪松夏季核心时钟基因和转录组的昼夜节律。冬季昼夜节律的减弱表明昼夜节律随环境条件而发生季节性变化。这些数据还揭示了与被子植物相比表现出不同表达模式的基因，这表明针叶树中存在着独特的基因调控网络。本研究为了解针叶树转录调控机制提供了基础数据。
BackgroundThe circadian clock and diurnal dynamics of the transcriptome are presumed to play important roles in the regulation of physiological, biological and developmental processes synchronized with diurnal and annual cycles of plant environments. However, little is known about the circadian clock and its regulation in gymnosperms, including conifers. Here we present the diurnal transcriptome dynamics of Japanese cedar (Cryptomeria japonica (L.f.) D.Don) in both active (summer) and dormant (winter) periods.ResultsMicroarray analysis revealed significant differences in transcripts between summer and winter, and diurnal transcriptome dynamics only in the summer. About 7.7% of unique genes (556 out of 7,254) on the microarray were periodically expressed in summer. Expression patterns of some genes, especially light-related genes, did not show significant oscillation in Japanese cedar, thus differing from those reported in angiosperms. Gene network analysis of the microarray data revealed a network associated with the putative core clock genes (CjLHYa, CjLHYb, CjTOC1, CjGI and CjZTL), which were also isolated, indicating their importance in the diurnal regulation of the transcriptome.ConclusionThis study revealed the existence of core clock genes and diurnal rhythms of the transcriptome in summer in Japanese cedar. Dampening of diurnal rhythms in winter indicated seasonal change in the rhythms according to environmental conditions. The data also revealed genes that showed different expression patterns compared to angiosperms, suggesting a unique gene regulatory network in conifers. This study provides fundamental data to understand transcriptional regulatory mechanisms in conifers.
影响因子：
56.9
作者：
Harmer, SL;Hogenesch, LB;Kay, SA
通讯作者：
Kay, SA
影响因子：
11.6
作者：
Wang, ZY;Kenigsbuch, D;Tobin, EM
通讯作者：
Tobin, EM