Three-dimensional structure of Rubella virus factories.
Three-dimensional structure of Rubella virus factories.
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DOI：
10.1016/j.virol.2010.06.043
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发表时间：
2010-09-30
期刊：
影响因子：
3.7
通讯作者：
Risco C
中科院分区：
文献类型：
--
作者：
Fontana J;López-Iglesias C;Tzeng WP;Frey TK;Fernández JJ;Risco C
病毒工厂是受感染细胞中的复杂结构，病毒在其中划分其生命周期。风疹病毒（RUBV）通过募集粗面内质网（RER）、线粒体和高尔基体围绕被称为细胞病变空泡或CPV的修饰的溶酶体组装工厂。这些细胞器含有活性复制复合物，将复制的RNA转移到高尔基体膜中的组装位点。利用电子断层扫描和冷冻断裂技术对RUBV工厂的三维结构进行了研究。CPV包含堆叠膜、刚性片、小囊泡和大液泡。这些膜是相互连接的，并与内吞途径沟通，因为它们包含内吞的BSA-金。RER和CPV通过蛋白质桥和紧密贴壁的膜偶联。高尔基体囊泡附着在CPV上，但与线粒体没有紧密接触。免疫金标记证实，线粒体蛋白p32是一个丰富的组成部分周围和内部的CPV，它可以发挥重要作用的工厂活动。
Viral factories are complex structures in the infected cell where viruses compartmentalize their life cycle. Rubella virus (RUBV) assembles factories by recruitment of rough endoplasmic reticulum (RER), mitochondria and Golgi around modified lysosomes known as cytopathic vacuoles or CPVs. These organelles contain active replication complexes that transfer replicated RNA to assembly sites in Golgi membranes. We have studied the structure of RUBV factory in three dimensions by electron tomography and freeze-fracture. CPVs contain stacked membranes, rigid sheets, small vesicles and large vacuoles. These membranes are interconnected and in communication with the endocytic pathway since they incorporate endocytosed BSA-gold. RER and CPVs are coupled through protein bridges and closely apposed membranes. Golgi vesicles attach to the CPVs but no tight contacts with mitochondria were detected. Immunogold labelling confirmed that the mitochondrial protein p32 is an abundant component around and inside CPVs where it could play important roles in factory activities.
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