Cellular electron microscopy imaging reveals the localization of the Hfq protein close to the bacterial membrane.
Cellular electron microscopy imaging reveals the localization of the Hfq protein close to the bacterial membrane.
复制标题
DOI：
10.1371/journal.pone.0008301
复制
发表时间：
2009-12-14
期刊：
影响因子：
3.7
通讯作者：
Risco C
中科院分区：
文献类型：
--
作者：
Diestra E;Cayrol B;Arluison V;Risco C
Hfq是一种细菌蛋白，参与了核酸交易的多个方面，但其最具特征的功能之一是通过与应激相关小调控（sRNA）的相互作用影响mRNA的转录后调控。通过电子显微镜下基于金属硫蛋白克隆标签的细胞成像，我们在这里证明了Hfq蛋白除了定位在细胞质和类核中外，还位于细胞周围。同时免疫金检测特异性标记强烈提示外周Hfq靠近细菌膜。由于sRNA调节几种膜蛋白的合成，我们的结果表明，这些蛋白的sRNA和hfq依赖的翻译调节发生在膜下的细胞质区域。这一发现支持了一种观点，即专门用于膜蛋白翻译的RNA加工和翻译机制可能经常位于靠近细菌细胞膜的位置。
Hfq is a bacterial protein involved in several aspects of nucleic acid transactions, but one of its best-characterized functions is to affect the post-transcriptional regulation of mRNA by virtue of its interactions with stress-related small regulatory (sRNA). By using cellular imaging based on the metallothionein clonable tag for electron microscopy, we demonstrate here that in addition to its localization in the cytoplasm and in the nucleoid, a significant amount of Hfq protein is located at the cell periphery. Simultaneous immunogold detection of specific markers strongly suggests that peripheral Hfq is close to the bacterial membrane. Because sRNAs regulate the synthesis of several membrane proteins, our result implies that the sRNA- and Hfq-dependent translational regulation of these proteins takes place in the cytoplasmic region underlying the membrane. This finding supports the proposal that RNA processing and translational machineries dedicated to membrane protein translation may often be located in close proximity to the membrane of the bacterial cell.
登录
查看更多内容
影响因子：
5.6
作者：
Arluison, V;Derreumaux, P;Régnier, P
通讯作者：
Régnier, P
影响因子：
10.5
作者：
Kawamoto, H;Morita, T;Aiba, H
通讯作者：
Aiba, H
DOI：
10.1016/j.bbrc.2005.02.025
发表时间：
2005-04-08
影响因子：
3.1
作者：
Jasiecki, J;Wegrzyn, G
通讯作者：
Wegrzyn, G
影响因子：
14.9
作者：
CASTLEMAN, H;MEREDITH, RD;ERLANGER, BF
通讯作者：
ERLANGER, BF
影响因子：
2.1
作者：
Azam, TA;Hiraga, S;Ishihama, A
通讯作者：
Ishihama, A