Different chain length specificity among three polyphosphate quantification methods.
Different chain length specificity among three polyphosphate quantification methods.
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三种多磷酸盐定量方法的链长特异性不同。
DOI：
10.1016/j.ab.2008.08.002
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发表时间：
2008
影响因子：
2.9
通讯作者：
M. Saito
中科院分区：
文献类型：
--
作者：
R. Ohtomo;Y. Sekiguchi;T. Kojima;M. Saito
多磷酸盐普遍存在于生物体中，具有多种生物化学功能。已知丛枝菌根真菌积累多磷酸盐作为其功能的关键化合物。然而，使用多磷酸激酶（PPK）的逆反应，其中多磷酸被转化为腺苷三磷酸（ATP）和荧光素酶测定定量的酶测定，未能检测到积累的多磷酸在一些菌根根。当将酵母外聚磷酸酶（PPX）应用于这些样品时，检测到比应用PPK测定时高得多的多磷酸盐水平。使用多磷酸盐链长标准品对这些方法的底物链长特异性的详细分析表明，PPX方法最适合检测短链多磷酸盐。可以以超过50%的效率定量的最短聚磷酸盐级分的平均链长对于PPX方法为3，对于PPK方法为38。PPK值与PPX值的比值可以作为比较不同样品中聚磷酸盐链长分布的一个简单而相对的指标。
Polyphosphate is ubiquitous among living organisms and has a variety of biochemical functions. Arbuscular mycorrhizal fungi have been known to accumulate polyphosphate as a key compound for their function. However, an enzymatic assay using polyphosphate kinase (PPK) reverse reaction, in which polyphosphate is converted to adenosine triphosphate (ATP) and quantified by luciferase assay, failed to detect accumulation of polyphosphate in some mycorrhizal root. When yeast exopolyphosphatase (PPX) was applied to these samples, a much higher polyphosphate level was detected than when the PPK assay was applied. Detailed analysis of substrate chain length specificity of these methods using polyphosphate chain length standards revealed that the PPX method was the most appropriate to detect short-chain polyphosphate. The average chain length of the shortest polyphosphate fraction that could be quantified with more than 50% efficiency was 3 for the PPX method and 38 for the PPK method. It was also suggested that the ratio of the PPK value to the PPX value may be useful as a simple and relative index to compare polyphosphate chain length distribution in different samples.
DOI：
10.1016/s0021-9258(19)38459-5
发表时间：
1990-07
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
K. Ahn;A. Kornberg
通讯作者：
K. Ahn;A. Kornberg
影响因子：
3.3
作者：
Ogawa, N;DeRisi, J;Brown, PO
通讯作者：
Brown, PO
用多磷酸葡萄糖激酶测定多磷酸盐的大小。
DOI：
--
发表时间：
1986
期刊：
Biochemistry international
影响因子：
--
作者：
Pepin,CA;Wood,HG;Robinson,NA
通讯作者：
Robinson,NA