Effects of Temperature on the Transmission of West Nile Virus by Culex tarsalis (Diptera: Culicidae)
Effects of Temperature on the Transmission of West Nile Virus by Culex tarsalis (Diptera: Culicidae)
复制标题
温度对跗库蚊（双翅目：库蚊科）传播西尼罗病毒的影响
DOI：
--
复制
发表时间：
2006
影响因子：
2.1
通讯作者：
V. Martinez
中科院分区：
文献类型：
--
作者：
W. Reisen;Ying Fang;V. Martinez
用西尼罗病毒（黄病毒科，黄病毒属，WNV）NY 99株感染跗库蚊（Culex tarsalis Coquillett）雌蚊，然后在10-30 ℃恒温下孵育。在选定的时间间隔，传输尝试使用体外毛细管测定。通过概率单位分析估计了从吸入感染性血粉到感染雌性传播西尼罗河病毒的中位时间（中位外源性潜伏期，EIP 50）。通过将EIP速率（EIP 50的倒数）作为14 - 30°C温度的函数进行回归，估计EIP需要109度-天（DD），病毒发展的零点（x截距）估计为14.3°C。由此产生的度日模型表明，NY 99 WNV毒株对温度的反应不同于来自南非的WNV谱系II毒株，并且接近我们先前对圣路易斯脑炎病毒（黄病毒科，黄病毒属，SLEV）的估计。因此，入侵的NY 99 WNV毒株需要温暖的温度才能有效传播。完成EIP的时间是根据2004年流行年期间在洛杉矶科切拉谷和加州克恩县记录的温度每月估计的，并与Cx的持续时间相关。跗跖生殖营养循环和WNV活性的措施。在温度升高后，地方性西尼罗河病毒活动开始，EIP的持续时间缩短，病毒可能在两个或更少的生殖营养循环中传播。2002-2004年流行夏季期间美国的温度表明，西尼罗河病毒传播和由此产生的震中与高于平均水平的夏季温度密切相关。
Abstract Culex tarsalis Coquillett females were infected with the NY99 strain of West Nile virus (family Flaviviridae, genus Flavivirus, WNV) and then incubated under constant temperatures of 10–30°C. At selected time intervals, transmission was attempted using an in vitro capillary tube assay. The median time from imbibing an infectious bloodmeal until infected females transmitted WNV (median extrinsic incubation period, EIP50) was estimated by probit analysis. By regressing the EIP rate (inverse of EIP50) as a function of temperature from 14 to 30°C, the EIP was estimated to require 109 degree-days (DD) and the point of zero virus development (x-intercept) was estimated to be 14.3°C. The resulting degree-day model showed that the NY99 WNV strain responded to temperature differently than a lineage II strain of WNV from South Africa and approximated our previous estimates for St. Louis encephalitis virus (family Flaviviridae, genus Flavivirus, SLEV). The invading NY99 WNV strain therefore required warm temperatures for efficient transmission. The time for completion of the EIP was estimated monthly from temperatures recorded at Coachella Valley, Los Angeles, and Kern County, California, during the 2004 epidemic year and related to the duration of the Cx. tarsalis gonotrophic cycle and measures of WNV activity. Enzootic WNV activity commenced after temperatures increased, the duration of the EIP decreased, and virus potentially was transmitted in two or less gonotrophic cycles. Temperatures in the United States during the epidemic summers of 2002–2004 indicated that WNV dispersal and resulting epicenters were linked closely to above-average summer temperatures.
温度对跗库蚊（双翅目：库蚊科）传播西方马脑脊髓炎和圣路易斯脑炎病毒的影响。
影响因子：
2.1
作者：
Reisen,WK;Meyer,RP;Presser,SB;Hardy,JL
通讯作者：
Hardy,JL